Identification of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) by using polymerase chain reaction amplification and restriction analysis of the mitochondrial cytochrome b gene

被引：39

作者：

Carrera, E ^{[1
]}

Garcia, T ^{[1
]}

Cespedes, A ^{[1
]}

Gonzalez, I ^{[1
]}

Sanz, B ^{[1
]}

Hernandez, PE ^{[1
]}

Martin, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol 3, E-28040 Madrid, Spain

来源：

JOURNAL OF FOOD PROTECTION | 1998年 / 61卷 / 04期

关键词：

D O I：

10.4315/0362-028X-61.4.482

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the identification of fresh and smoked samples of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss). Digestion of the 359-bp PCR product with the endonucleases EcoRV and TaqI yielded specific banding patterns for salmon and trout. This genetic marker can be very useful for detecting fraudulent substitution of the cheaper smoked trout for the more expensive smoked salmon.

引用

页码：482 / 486

页数：5

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