Fluorescence aptasensor based on competitive-binding for human neutrophil elastase detection

被引：27

作者：

He, Jing-Lin ^{[1
]}

Wu, Zai-Sheng ^{[1
]}

Zhang, Song-Bai ^{[1
]}

Shen, Guo-Li ^{[1
]}

Yu, Ru-Qin ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

来源：

TALANTA | 2010年 / 80卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Aptasensor; Competitive-binding reaction; Human neutrophil elastase; Molecular beacon; Fluorescence; MOLECULAR-INTERACTIONS; IMMUNITY; BEACONS; DNA;

D O I：

10.1016/j.talanta.2009.09.019

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

To our knowledge, we report the first fluorescence aptasensor for detecting human neutrophil elastase (HNE) in homogeneous solution. The biosensor contains a short DNA scrambled sequence strand (SS) complementary to part of the aptamer sequence or the loop of molecular beacon (MB). The aptamer-HNE recognition event involves competition between the molecular beacon and loose HNE aptamer for the binding the short DNA strand. The new biosensor can detect as little as 0.34 nM of HNE, and the response is linear in the tested concentration range of 0.34-68 nM with the detection limit of 47 pM. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：1264 / 1268

页数：5

共 14 条

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Birrer P, 1993, Agents Actions Suppl, V40, P3

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