Functional identification of the pro-apoptotic effector domain in human Sox4

被引：36

作者：

Hur, EH

Hur, W

Choi, JY

Kim, IK

Kim, HY

Yoon, SK ^{[1
]}

Rhim, H

机构：

[1] Catholic Univ Korea, Res Inst Mol Genet, Coll Med, Catholic Res Inst Med Sci, Seoul 137701, South Korea

[2] Catholic Univ Korea, Dept Biomed Sci, Coll Med, Seoul 137701, South Korea

[3] Catholic Univ Korea, Coll Med, Dept Internal Med, Seoul 137701, South Korea

[4] Catholic Univ Korea, Coll Med, Dept Biochem, Seoul 137701, South Korea

[5] Catholic Univ Korea, Ctr Rheumat Dis, Kangnam St Marys Hosp, Coll Med, Seoul 137701, South Korea

[6] Catholic Univ Korea, WHO, Collaborating Ctr Viral Hepatitis, Coll Med, Seoul 137701, South Korea

[7] Sangmyung Univ, Dept Biol, Seoul 110743, South Korea

[8] Univ Ulsan, Coll Med, Dept Med, Seoul 138736, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 325卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Sox4; glycine-rich region; high mobility group; serine-rich region; DNA-binding domain; trans-activation domain; apoptosis;

D O I：

10.1016/j.bbrc.2004.09.215

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies provide evidence that Sox4 is involved in regulating apoptosis as well as tumorigenesis of various human cancers; however, its role in the apoptotic machinery is not fully understood. Here we describe that the central domain containing glycine-rich region in Sox4, named CD, is a pivotal pro-apoptotic domain to induce apoptotic cell death. Deletion of the DNA-binding domain or trans-activation domain in Sox4 did not significantly affect pro-apoptotic activity, whereas transient transfection of the high mobility group box or the serine-rich region abrogated the apoptotic activity. Moreover, overexpression of the CD construct (aa 166-342) revealed the apoptotic activity comparable to that of wild-type Sox4, approximately 60%, of cell death. Our data suggest that the apoptotic activity of Sox4 can be dissociated from its transcriptional trans-activation and is mediated through its CD. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：59 / 67

页数：9

共 42 条

[1] Identification of cDNAs for Sox-4, an HMG-Box protein, and a novel human homolog of yeast splicing factor SSF-1 differentially regulated during apoptosis induced by prostaglandin A2/Δ12-PGJ2 in Hep3B cells [J].