Rare genetic variation at Zea mays crtRB1 increases β-carotene in maize grain

被引：366

作者：

Yan, Jianbing ^{[2
,3
,4
]}

Kandianis, Catherine Bermudez ^{[1
,5
]}

Harjes, Carlos E. ^{[4
,12
]}

Bai, Ling ^{[6
]}

Kim, Eun-Ha ^{[7
]}

Yang, Xiaohong ^{[3
]}

Skinner, Debra J. ^{[1
,5
]}

Fu, Zhiyuan ^{[3
]}

Mitchell, Sharon ^{[4
]}

Li, Qing ^{[3
]}

Fernandez, Maria G. Salas ^{[4
,8
]}

Zaharieva, Maria ^{[2
]}

Babu, Raman ^{[2
]}

Fu, Yang ^{[1
,3
,5
]}

Palacios, Natalia ^{[2
]}

Li, Jiansheng ^{[3
]}

DellaPenna, Dean ^{[7
]}

Brutnell, Thomas ^{[6
]}

Buckler, Edward S. ^{[4
,9
,10
]}

Warburton, Marilyn L. ^{[2
,11
]}

Rocheford, Torbert ^{[1
,5
]}

机构：

[1] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA

[2] Int Maize & Wheat Improvement Ctr CIMMYT, Texcoco, Mexico

[3] China Agr Univ, Natl Maize Improvement Ctr China, Beijing 100094, Peoples R China

[4] Cornell Univ, Inst Genom Div, Ithaca, NY USA

[5] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA

[6] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA

[7] Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA

[8] Iowa State Univ, Dept Agron, Ames, IA USA

[9] USDA ARS, Plant Soil & Nutr Res Unit, Ithaca, NY 14853 USA

[10] Cornell Univ, Dept Genet & Plant Breeding, Ithaca, NY USA

[11] USDA ARS, Corn Host Plant Resistance Res Unit, Starkville, MS USA

[12] Monsanto Co, Leesburg, GA USA

来源：

NATURE GENETICS | 2010年 / 42卷 / 04期

基金：

美国国家科学基金会;

关键词：

BIOFORTIFICATION; BIOSYNTHESIS; ARABIDOPSIS; HYDROXYLASE; PROVITAMIN; GERMPLASM; LOCUS; CROPS;

D O I：

10.1038/ng.551

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Breeding to increase beta-carotene levels in cereal grains, termed provitamin A biofortification, is an economical approach to address dietary vitamin A deficiency in the developing world. Experimental evidence from association and linkage populations in maize (Zea mays L.) demonstrate that the gene encoding beta-carotene hydroxylase 1 (crtRB1) underlies a principal quantitative trait locus associated with beta-carotene concentration and conversion in maize kernels. crtRB1 alleles associated with reduced transcript expression correlate with higher beta-carotene concentrations. Genetic variation at crtRB1 also affects hydroxylation efficiency among encoded allozymes, as observed by resultant carotenoid profiles in recombinant expression assays. The most favorable crtRB1 alleles, rare in frequency and unique to temperate germplasm, are being introgressed via inexpensive PCR marker-assisted selection into tropical maize germplasm adapted to developing countries, where it is most needed for human health.

引用

页码：322 / U74

页数：8

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