Cloning and characterization of the 5′-flanking region of the human cardiotrophin-1 gene

被引：8

作者：

Erdmann, J

Hassfeld, S

Kallisch, H

Fleck, E

Regitz-Zagrosek, V

机构：

[1] Humboldt Univ, Klinikum Rudolf Virchow, Dept Internal Med Cardiol, D-13353 Berlin, Germany

[2] Deutsch Herzzentrum Berlin, D-13353 Berlin, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 244卷 / 02期

关键词：

cardiotrophin-1 (CT-1); promoter; reportergene assay; COS-7; cells;

D O I：

10.1006/bbrc.1998.8311

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To enable the analysis of the regulation of the human cardiotrophin-1 gene expression, the 5'-flanking region of the human cardiotrophin-1 gene was cloned and sequenced. Data bank search revealed several cis-active DNA elements (SP1, CREB, C/EBP, AP1 and AP2 like and GATA) in the proximal 1.1 kb region, Six nested 5-'terminal deletion mutants from -1091/+39 to -218/+39 were fused to a luciferase reportergene and proved to be functionally active after transfection into COS-7 cells. (C) 1998 Academic Press.

引用

收藏

页码：494 / 497

页数：4

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