Multicenter validation of the cppB gene as a PCR target for detection of Neisseria gonorrhoeae

被引:31
作者
Bruisten, SM
Noordhoek, GT
van den Brule, AJC
Duim, B
Boel, CHE
El-Faouzi, K
du Maine, R
Mulder, S
Luijt, D
Schirm, J
机构
[1] GG&GD, Municipal Hlth Serv, Publ Hlth Lab, NL-1018 WT Amsterdam, Netherlands
[2] AMC, Dept Med Microbiol, Amsterdam, Netherlands
[3] LVF, Publ Hlth Lab Friesland, Leeuwarden, Netherlands
[4] PAMM, Lab Pathol & Microbiol, Veldhoven, Netherlands
[5] SLGD, Reg Publ Hlth Lab, Groningen, Netherlands
关键词
D O I
10.1128/JCM.42.9.4332-4334.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The cppB gene is often used as a target for detection of Neisseria gonorrhoeae by PCR. Using a coded panel of 500 DNA samples, we determined that the cppB gene is missing in 5.8% of N. gonorrhoeae strains, and therefore we consider the cppB gene to be an unsuitable target.
引用
收藏
页码:4332 / 4334
页数:3
相关论文
共 14 条
[11]   ECOLOGY OF GONOCOCCAL PLASMIDS [J].
ROBERTS, M ;
PIOT, P ;
FALKOW, S .
JOURNAL OF GENERAL MICROBIOLOGY, 1979, 114 (OCT) :491-494
[12]   Comparison between the LCx probe system and the COBAS AMPLICOR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae infections in patients attending a clinic for treatment of sexually transmitted diseases in Amsterdam, The Netherlands [J].
van Doornum, GJJ ;
Schouls, LM ;
Pijl, A ;
Cairo, I ;
Buimer, M ;
Bruisten, S .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (03) :829-835
[13]   Evaluation of the Roche Neisseria gonorrhoeae 16S rRNA PCR for confirmation of AMPLICOR PCR-positive samples and comparison of its diagnostic performance according to storage conditions and preparation of endocervical specimens [J].
Van Dyck, E ;
Smet, H ;
Van Damme, L ;
Laga, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (06) :2280-2282
[14]   Detection of Neisseria meningitidis by LightCycler PCR [J].
Whiley, DM ;
Crisante, ME ;
Syrmis, MW ;
Mackay, IM ;
Sloots, TP .
PATHOLOGY, 2003, 35 (04) :347-349