Two distinct ospA genes among Borrelia valaisiana strains

被引:19
作者
Wang, GQ [1 ]
van Dam, AP [1 ]
Danker, J [1 ]
机构
[1] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, NL-1105 AZ Amsterdam, Netherlands
关键词
Lyme disease; Borrelia burgdorferi; Borrelia valaisiana; OspA;
D O I
10.1016/S0923-2508(00)00155-8
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Borrelia valaisiana is a recently described bacterial species in the Borrelia burgdorferi sensu late complex. To further characterize this bacterium, the plasmid-encoded ospA genes from eight B. valaisiana isolates were amplified by PCR, cloned and sequenced. All B. valaisiana isolates studied possessed an ospA gene with a size of 822-825 bp. The identity of the predicted amino acid sequences of the OspA proteins among B. valaisiana isolates was 69.1-100%, and ranged from 68.2 to 79.1% between B. valaisiana and other B. burgdorferi sensu late species. Based on the OspA protein sequences, the eight B. valaisiana isolates could be distinguished into two subgroups. Subgroup I contained six B, valaisiana isolates of which OspA sequences were almost identical, but clearly differed from other LB spirochetes. Subgroup II consisted of two isolates with identical OspA sequences which were only 70% identical to subgroup I B. valaisiana isolates and similarly distant from the OspA sequences of other B. burgdorferi sensu late genospecies. Phylogenetic analysis indicates that B. valaisiana isolates belonging to subgroups I and II possibly evolved from two distinct ancestors. Our data showed for the first time a major difference in OspA proteins within a well-defined B. burgdorferi sensu late species at the evolutionary level, suggesting that it is not always reliable to assign Borrelia isolates to a definite species solely based on data from ospA gene sequence analysis. (C) 2000 Editions scientifiques et medicales Elsevier SAS.
引用
收藏
页码:325 / 331
页数:7
相关论文
共 36 条
[1]   DELINEATION OF BORRELIA-BURGDORFERI SENSU-STRICTO, BORRELIA-GARINII SP-NOV, AND GROUP VS461 ASSOCIATED WITH LYME BORRELIOSIS [J].
BARANTON, G ;
POSTIC, D ;
SAINTGIRONS, I ;
BOERLIN, P ;
PIFFARETTI, JC ;
ASSOUS, M ;
GRIMONT, PAD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (03) :378-383
[2]   MONOCLONAL-ANTIBODIES FOR IDENTIFICATION OF BORRELIA-AFZELII SP-NOV - ASSOCIATED WITH LATE CUTANEOUS MANIFESTATIONS OF LYME BORRELIOSIS [J].
CANICA, MM ;
NATO, F ;
DUMERLE, L ;
MAZIE, JC ;
BARANTON, G ;
POSTIC, D .
SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 1993, 25 (04) :441-448
[3]   LINEAR CHROMOSOMES OF LYME-DISEASE AGENT SPIROCHETES - GENETIC DIVERSITY AND CONVERSATION OF GENE ORDER [J].
CASJENS, S ;
DELANGE, M ;
LEY, HL ;
ROSA, P ;
HUANG, WM .
JOURNAL OF BACTERIOLOGY, 1995, 177 (10) :2769-2780
[4]   Rate of infection of Ixodes ricinus ticks with Borrelia burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii and group VS116 in an endemic focus of lyme disease in Italy [J].
Cinco, M ;
Padovan, D ;
Murgia, R ;
Poldini, L ;
Frusteri, L ;
van de Pol, I ;
Verbeek-De Kruif, N ;
Rijpkema, S ;
Maroli, M .
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1998, 17 (02) :90-94
[5]   SIMULTANEOUS PRESENCE OF DIFFERENT BORRELIA-BURGDORFERI GENOSPECIES IN BIOLOGICAL-FLUIDS OF LYME-DISEASE PATIENTS [J].
DEMAERSCHALCK, I ;
BENMESSAOUD, A ;
DEKESEL, M ;
HOYOIS, B ;
LOBET, Y ;
HOET, P ;
BIGAIGNON, G ;
BOLLEN, A ;
GODFROID, E .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :602-608
[6]   Borrelia burgdorferi genes selectively expressed in ticks and mammals [J].
deSilva, AM ;
Fikrig, E .
PARASITOLOGY TODAY, 1997, 13 (07) :267-270
[7]   BORRELIA-BURGDORFERI IS CLONAL - IMPLICATIONS FOR TAXONOMY AND VACCINE DEVELOPMENT [J].
DYKHUIZEN, DE ;
POLIN, DS ;
DUNN, JJ ;
WILSKE, B ;
PREACMURSIC, V ;
DATTWYLER, RJ ;
LUFT, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (21) :10163-10167
[8]  
Eiffert H, 1998, SCAND J INFECT DIS, V30, P265, DOI 10.1080/00365549850160918
[9]   PROTECTION OF MICE AGAINST THE LYME-DISEASE AGENT BY IMMUNIZING WITH RECOMBINANT OSPA [J].
FIKRIG, E ;
BARTHOLD, SW ;
KANTOR, FS ;
FLAVELL, RA .
SCIENCE, 1990, 250 (4980) :553-556
[10]   Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi [J].
Fraser, CM ;
Casjens, S ;
Huang, WM ;
Sutton, GG ;
Clayton, R ;
Lathigra, R ;
White, O ;
Ketchum, KA ;
Dodson, R ;
Hickey, EK ;
Gwinn, M ;
Dougherty, B ;
Tomb, JF ;
Fleischmann, RD ;
Richardson, D ;
Peterson, J ;
Kerlavage, AR ;
Quackenbush, J ;
Salzberg, S ;
Hanson, M ;
vanVugt, R ;
Palmer, N ;
Adams, MD ;
Gocayne, J ;
Weidman, J ;
Utterback, T ;
Watthey, L ;
McDonald, L ;
Artiach, P ;
Bowman, C ;
Garland, S ;
Fujii, C ;
Cotton, MD ;
Horst, K ;
Roberts, K ;
Hatch, B ;
Smith, HO ;
Venter, JC .
NATURE, 1997, 390 (6660) :580-586