Expression of a plant defensin in rice confers resistance to fungal phytopathogens

被引:103
作者
Jha, Sanjay [1 ,2 ]
Chattoo, Bharat B. [1 ,2 ]
机构
[1] Maharaja Sayajirao Univ Baroda, Fac Sci, Dept Microbiol, Ctr Genome Res, Vadodara 390002, Gujarat, India
[2] Maharaja Sayajirao Univ Baroda, Fac Sci, Ctr Biotechnol, Vadodara 390002, Gujarat, India
关键词
Rs-AFP2; Magnaporthe oryzae; Rhizoctonia solani; Transgenic rice; Plant-microbe interactions; SHOWS ENHANCED RESISTANCE; ANTIFUNGAL PROTEINS; VECTORS; OVEREXPRESSION; TRANSFORMATION; AGROBACTERIUM; RADISH; LEVEL; GENE;
D O I
10.1007/s11248-009-9315-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Transgenic rice (Oryza sativa L. cv. Pusa basmati 1), overexpressing the Rs-AFP2 defensin gene from the Raphanus sativus was generated by Agrobacterium tumefaciens-mediated transformation. Expression levels of Rs-AFP2 ranged from 0.45 to 0.53% of total soluble protein in transgenic plants. It was observed that constitutive expression of Rs-AFP2 suppresses the growth of Magnaporthe oryzae and Rhizoctonia solani by 77 and 45%, respectively. No effect on plant morphology was observed in the Rs-AFP2 expressing rice lines. The inhibitory activity of protein extracts prepared from leaves of Rs-AFP2 plants on the in vitro growth of M. oryzae indicated that the Rs-AFP2 protein produced by transgenic rice plants was biologically active. Transgene expression of Rs-AFP2 was not accompanied by an induction of pathogenesis-related (PR) gene expression, suggesting that the expression of Rs-AFP2 directly inhibits the pathogens. Here, we demonstrate that transgenic rice plants expressing the Rs-AFP2 gene show enhanced resistance to M. oryzae and R. solani, two of the most important pathogens of rice.
引用
收藏
页码:373 / 384
页数:12
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