Enzymatic profiling system in a small-molecule microarray

被引：108

作者：

Zhu, Q

Uttamchandani, M

Li, DB

Lesaicherre, ML

Yao, SQ

机构：

[1] Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore

[2] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

ORGANIC LETTERS | 2003年 / 5卷 / 08期

关键词：

D O I：

10.1021/ol034233h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We have developed a microarray-based strategy for detection of three major classes of hydrolytic enzymes on the basis of their catalytic activities. This enables the sensitive detection of proteins not merely by their bindings but rather by their enzymatic activities. This may provide a valuable tool for screening, identification, and characterization of new enzymes in a high-throughput fashion.

引用

收藏

页码：1257 / 1260

页数：4

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