In vivo magnetic resonance imaging of iron oxide-labeled, arterially-injected mesenchymal stem cells in kidneys of rats with acute ischemic kidney injury:: Detection and monitoring at 3T

被引:80
作者
Ittrich, Harald
Lange, Claudia
Toegel, Florian
Zander, Axel R.
Dahnke, Hannes
Westenfelder, Christof
Adam, Gerhard
Nolte-Ernsting, Claus
机构
[1] Univ Hamburg Eppendorf, Med Ctr, Dept Diagnost & Intervent Radiol, D-20246 Hamburg, Germany
[2] Univ Hamburg Eppendorf, Med Ctr, Dept Haematol Oncol Bone Marrow Transplantat, D-20246 Hamburg, Germany
[3] Univ Utah, Dept Med, Div Nephrol, Salt Lake City, UT 84112 USA
[4] Philips Res Europe Hamubrg, Sector Med Imaging Syst, Hamburg, Germany
[5] VA Med Ctr, Salt Lake City, UT USA
[6] Univ Utah, Dept Physiol, Salt Lake City, UT 84112 USA
关键词
MRI; cell tracking; mesenchymal stem cells; iron oxide particles; acute kidney injury;
D O I
10.1002/jmri.20925
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Purpose: To evaluate MRI for a qualitative and quantitative in vivo tracking of intraaortal injected iron oxide-labeled mesenchymal stem cells (MSC) into rats with acute kidney injury (AKI). Materials and Methods: In vitro MRI and R-2* measurement of nonlabeled and superparamagnetic iron oxide (SPIO)-labeled MSC (MSCSPIO) was performed in correlation, to cellular iron content and cytological examination (Prussian blue, electron microscopy). In vivo MRI and R2* evaluation were performed before and after ischemic/reperfusion AKI (N = 14) and intraaortal injection of 1.5 X 10(6) MSCSPIO (N = 7), fetal calf serum (FCS) (medium, N = 6), and SPIO alone (N = 1) up to 14 days using a clinical 3T scanner. Signal to noise ratios (SNR), R2* of kidneys, liver, spleen, and bone marrow, renal function (creatinine [CREA], blood urea nitrogen [BUN)), and kidney volume were measured and tested for statistical significance (Student's t-test, P < 0.05) in comparison histology (hematoxylin and eosin [H&E], Prussian blue, periodic acid-Schiff [PAS], CD68). Results: In vitro, MSCSPIO showed a reduction of SNR and T-2* with R-2* approximate to number of MSCSPIO) (R-2 = 0.98). In vivo MSCspIo administration resulted in a SNR decrease (35 +/- 15%) and R-2* increase (101 +/- 18.3%) in renal cortex caused by MSCSPIO accumulation in contrast to control animals, (P < 0.01). Liver, spleen, and bone marrow (MSCSPIO) showed a delayed SNR decline/R-2* increase (P < from MSCSPIO migration. The increase of kidney volume and the decrease in renal function (P < 0.05) was reduced in MSC-treated animals. Conclusion: Qualitative and quantitative in vivo cell-tracking and monitoring of organ distribution of intraaortal injected MSCSPIO in AKI is feasible in MRI at 3T.
引用
收藏
页码:1179 / 1191
页数:13
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