DNA methylation pattern in human zygotes and developing embryos

被引:180
作者
Fulka, H
Mrazek, M
Tepla, O
Fulka, J [1 ]
机构
[1] VUZV Labs, Ctr Cell Therapy & Tissue Repair, Prague 10, Czech Republic
[2] ISCARE IVF, Prague 9, Czech Republic
关键词
D O I
10.1530/rep.1.00217
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report on observations of the global methylation/demethylation pattern of both pronuclei in human zygotes and in early embryos up to the blastocyst stage. Our results demonstrate that in about half of the zygotes examined the paternal chromatin was less methylated than the maternal chromatin. In the other half, both pronuclei exhibited the same intensity of labeling. The nuclei in developing embryos were intensively labeled for up to the four-cell stage; thereafter, a decline of labeling intensity was detected. Remethylation in some nuclei starts in late morulae. Surprisingly, and unlike the mouse, at the blastocyst stage the inner cell mass showed a weaker intensity of labeling than the trophectodermal cells.
引用
收藏
页码:703 / 708
页数:6
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