Reevaluation of the Premier Clostridium difficile toxin A and B immunoassay with comparison to glutamate dehydrogenase common antigen testing evaluating Bartels cytotoxin and Prodesse ProGastro™ Cd polymerase chain reaction as confirmatory procedures

被引：26

作者：

Doing, Kirk M. ^{[1
,2
]}

Hintz, Marilyn S. ^{[1
]}

Keefe, Calvin ^{[1
]}

Horne, Sarah ^{[2
]}

LeVasseur, Shelby ^{[2
]}

Kulikowski, Martha L. ^{[1
]}

机构：

[1] Affiliated Lab Inc, Bangor, ME 04401 USA

[2] Univ Maine, Dept Biol & Ecol, Orono, ME 04469 USA

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2010年 / 66卷 / 02期

关键词：

Clostridium difficile; Toxin; REAL-TIME PCR; ENZYME-IMMUNOASSAY; LABORATORY DIAGNOSIS; FECAL SPECIMENS; DIARRHEA; DISEASE; ASSAY; ALGORITHM; CULTURE; EPIDEMIC;

D O I：

10.1016/j.diagmicrobio.2009.09.001

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Enzyme immunoassays are currently the most common tests used in the clinical laboratory for the detection of Clostridium difficile toxins; however, significant problems with their performance have recently been described. We prospectively reevaluated the Meridian Premier C. difficile toxin A/B assay with direct comparison to a 2-step algorithm that screened for C. difficile common antigen and compared cytotoxin and real-time polymerase chain reaction (PCR) as confirmatory procedures. The Premier assay lacked sufficient sensitivity, missing 25% of true-positive samples. PCR was the most sensitive method and the only procedure that allowed same day testing and reporting. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：129 / 134

页数：6

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