Kinetics of Neutralizing Antibodies in Patients Naturally Infected by H5N1 Virus

被引:61
作者
Buchy, Philippe [1 ]
Vong, Sirenda [1 ]
Chu, Simon [1 ]
Garcia, Jean-Michel [2 ]
Tran Tinh Hien [3 ,4 ]
Vo Minh Hien [3 ,4 ]
Channa, Mey [1 ]
Do Quang Ha [4 ]
Nguyen Van Vinh Chau [4 ]
Simmons, Cameron [4 ]
Farrar, Jeremy J. [4 ]
Peiris, Malik [2 ,5 ]
de Jong, Menno D. [4 ,6 ]
机构
[1] Inst Pasteur Cambodia, Phnom Penh, Cambodia
[2] HKU Pasteur Res Ctr, Hong Kong, Hong Kong, Peoples R China
[3] Hosp Trop Dis, Ho Chi Minh City, Vietnam
[4] Univ Oxford, Clin Res Unit, Ho Chi Minh City, Vietnam
[5] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
[6] Univ Amsterdam, Acad Med Ctr, Dept Med Microbiol, NL-1105 AZ Amsterdam, Netherlands
来源
PLOS ONE | 2010年 / 5卷 / 05期
基金
英国惠康基金;
关键词
INFLUENZA-A H5N1; POULTRY WORKERS; LOW-FREQUENCY; TRANSMISSION; THAILAND;
D O I
10.1371/journal.pone.0010864
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Little is known about the kinetics of anti-H5 neutralizing antibodies in naturally H5N1-infected patients with severe clinical illness or asymptomatic infection. Methods: Using H5N1 microneutralisation (MN) and H5-pseudotype particle-based microneutralisation assays (H5pp) we analyzed sera sequentially obtained from 11 severely ill patients diagnosed by RT-PCR (follow-up range 1-139 weeks of disease onset) and 31 asymptomatically infected individuals detected in a sero-epidemiological study after exposure to H5N1 virus (follow-up range: 1-2 month -11 months after exposure). Results: Of 44 sera from 11 patients with H5N1 disease, 70% tested positive by MN (antibody titre >= 80) after 2 weeks and 100% were positive by 3 weeks after disease onset. The geometric mean MN titers in severely ill patients were 540 at 1-2 months and 173 at 10-12 months and thus were higher than the titers from asymptomatic individuals (149 at 1-2 months, 62.2 at 10-12 months). Fractional polynomial regression analysis demonstrated that in all severely ill patients, positive titers persisted beyond 2 years of disease onset, while 10 of 23 sera collected 10-11 months after exposure in asymptomatically infected individuals tested negative. Conclusions: Our results indicate that people with asymptomatic H5N1 infection have lower H5N1 antibody titres compared to those with severe illness and that in many asymptomatically infected patients the antibody titer decreased to levels below the threshold of positivity within one year. These data are essential for the design and interpretation of sero-epidemiological studies.
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页数:7
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  • [21] Reproducibility of Serologic Assays for Influenza Virus A (H5N1)
    Stephenson, Iain
    Heath, Alan
    Major, Diane
    Newman, Robert W.
    Hoschler, Katja
    Junzi, Wang
    Katz, Jacqueline M.
    Weir, Jerry P.
    Zambon, Maria C.
    Wood, John M.
    [J]. EMERGING INFECTIOUS DISEASES, 2009, 15 (08) : 1250 - 1259
  • [22] A comparison of the pathogenicity of avian and swine H5N1 influenza viruses in Indonesia
    Takano, Ryo
    Nidom, Chairul A.
    Kiso, Maki
    Muramoto, Yukiko
    Yamada, Shinya
    Shinya, Kyoko
    Sakai-Tagawa, Yuko
    Kawaoka, Yoshihiro
    [J]. ARCHIVES OF VIROLOGY, 2009, 154 (04) : 677 - 681
  • [23] Swine Influenza Viruses: A North American Perspective
    Vincent, Amy L.
    Ma, Wenjun
    Lager, Kelly M.
    Janke, Bruce H.
    Richt, Juergen A.
    [J]. ADVANCES IN VIRUS RESEARCH, VOL 72, 2008, 72 : 127 - 154
  • [24] Low frequency of poultry-to-human H5NI virus transmission, Southern Cambodia, 2005
    Vong, Sirenda
    Coghlan, Benjamin
    Mardy, Sek
    Holl, Davun
    Seng, Heng
    Ly, Sovann
    Miller, Megge J.
    Buchy, Philippe
    Froehlich, Yves
    Dufourcq, Jean Baptiste
    Uyeki, Timothy M.
    Lim, Wilina
    Sok, Touch
    [J]. EMERGING INFECTIOUS DISEASES, 2006, 12 (10) : 1542 - 1547
  • [25] Risk Factors Associated with Subclinical Human Infection with Avian Influenza A (H5N1) Virus-Cambodia, 2006
    Vong, Sirenda
    Ly, Sowath
    Van Kerkhove, Maria D.
    Achenbach, Jenna
    Holl, Davun
    Buchy, Philippe
    Sorn, San
    Seng, Heng
    Uyeki, Timothy M.
    Sok, Touch
    Katz, Jacqueline M.
    [J]. JOURNAL OF INFECTIOUS DISEASES, 2009, 199 (12) : 1744 - 1752
  • [26] WHO, REC LAB PROC DET AV