Optimization of ultrasound-assisted extraction of Lingzhi polysaccharides using response surface methodology and its inhibitory effect on cervical cancer cells

被引：136

作者：

Chen, XiaoPing ^{[1
]}

Wang, WanXiang ^{[2
]}

Li, ShuiBing ^{[3
]}

Xue, JinLing ^{[1
]}

Fan, LiJun ^{[1
]}

Sheng, ZhongJi ^{[4
]}

Chen, YouGuo ^{[4
]}

机构：

[1] YanCheng 1th People Hosp, Dept Obstet & Gynecol, Yancheng City 224000, Jiangsu, Peoples R China

[2] YanCheng 1th People Hosp, Dept Sci Educ, Yancheng City 224000, Jiangsu, Peoples R China

[3] YanCheng 1th People Hosp, Dept Facil, Yancheng City 224000, Jiangsu, Peoples R China

[4] Soochow Univ, Dept Obstet & Gynecol, Affiliated Hosp 1, Suzhou 215006, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 80卷 / 03期

关键词：

Lingzhi polysaccharides; Ultrasonic-assisted extraction; Optimization; Response surface methodology (RSM); CaSki; HeLa; GANODERMA-LUCIDUM; PROCESS PARAMETERS; ACID;

D O I：

10.1016/j.carbpol.2010.01.010

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

In this work, an ultrasound-assisted procedure for the extraction of Lingzhi polysaccharides was established. Response surface methodology (RSM) was used to optimize the ultrasound-assisted extraction parameters (ultrasonic frequency (X1), extraction temperature (X2), extraction time (X3), and ratio of water to raw material (X4)) for enhancing the forward extraction efficiency of Lingzhi polysaccharides by implementing a three-level, four-variable Box-Behnken experimental design. The independent variable with the largest effect on response was X1 (ultrasonic frequency), followed by X2 (extraction temperature) and X3 (extraction time). The optimum extraction conditions were found to be ultrasonic frequency 8 kHz, extraction temperature 95 degrees C, extraction time 3 h and ratio of water to raw material 12. Under these conditions, the forward extraction efficiency of Lingzhi polysaccharides can increase from 42% to 75%. Pharmacological experiment showed that Lingzhi polysaccharides could decrease CyclinB1 mRNA expression in CaSki cells and inhibit CaSki and HeLa cells proliferation. Results indicated that Lingzhi polysaccharides possessed strong antitumor activity. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：944 / 948

页数：5

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