Measuring gene expression by quantitative proteome analysis

被引：115

作者：

Gygi, SP ^{[1
]}

Rist, B ^{[1
]}

Aebersold, R ^{[1
]}

机构：

[1] Univ Washington, Dept Mol Biotechnol, Seattle, WA 98195 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2000年 / 11卷 / 04期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/S0958-1669(00)00116-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteome analysis is most commonly accomplished by the combination of two-dimensional gel electrophoresis for protein separation, visualization, and quantification and mass spectrometry for protein identification. Over the past year, exceptional progress has been made towards developing a new technology base for the precise quantification and identification of proteins in complex mixtures, that is, quantitative proteomics.

引用

页码：396 / 401

页数：6

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