Biosensors and bio-bar code assays based on biofunctionalized magnetic microbeads

被引：104

作者：

Jaffrezic-Renault, Nicole ^{[1
]}

Martelet, Claude

Chevolot, Yann

Cloarec, Jean-Pierre

机构：

[1] Univ Lyon 1, LSA, F-69622 Villeurbanne, France

[2] Ecole Cent Lyon, AMPERE, F-69134 Ecully, France

[3] Ecole Cent Lyon, INL, F-69134 Ecully, France

来源：

SENSORS | 2007年 / 7卷 / 04期

关键词：

affinity biosensors; enzymatic biosensors; bio-bar code; magnetic microbeads;

D O I：

10.3390/s7040589

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review paper reports the applications of magnetic microbeads in biosensors and bio-bar code assays. Affinity biosensors are presented through different types of transducing systems: electrochemical, piezo electric or magnetic ones, applied to immunodetection and genodetection. Enzymatic biosensors are based on biofunctionalization through magnetic microbeads of a transducer, more often amperometric, potentiometric or conductimetric. The bio-bar code assays relie on a sandwich structure based on specific biological interaction of a magnetic microbead and a nanoparticle with a defined biological molecule. The magnetic particle allows the separation of the reacted target molecules from unreacted ones. The nanoparticles aim at the amplification and the detection of the target molecule. The bio-bar code assays allow the detection at very low concentration of biological molecules, similar to PCR sensitivity.

引用

页码：589 / 614

页数：26

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