Type III secretion system cluster 3 is required for maximal virulence of Burkholderia pseudomallei in a hamster infection model

被引：93

作者：

Warawa, J ^{[1
]}

Woods, DE ^{[1
]}

机构：

[1] Univ Calgary, Hlth Sci Ctr, Dept Microbiol & Infect Dis, Calgary, AB T2N 4N1, Canada

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 242卷 / 01期

基金：

加拿大健康研究院;

关键词：

melioidosis; Blurkholderia pseudomallei; type III secretion; animal studies; attenuation;

D O I：

10.1016/j.femsle.2004.10.045

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Burkholderia pseudoinallei, the etiological agent of melioidosis, is an animal pathogen capable of inducing a highly fatal septicemia. R pseudomallei possesses three type III secretion system (TTSS) clusters, two of which (TTSS I and TTSS2) are homologous to the TTSS of the plant pathogen Ralstonia solanacearum, and one (TTSS3) is homologous to the Salmonella SPI-I mammalian pathogenicity island. We have demonstrated that TTSS3 is required for the full virulence of B. pseudomallei in a hamster model of infection. We have also examined the virulence of R pseudomallei mutants deficient in several putative TTSS3 effector molecules, and found no significant attenuation of B. pseudomallei virulence in the hamster model. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：101 / 108

页数：8

共 33 条

[1] YSCU, A YERSINIA-ENTEROCOLITICA INNER MEMBRANE-PROTEIN INVOLVED IN YOP SECRETION [J].

ALLAOUI, A ;

WOESTYN, S ;

SLUITERS, C ;

CORNELIS, GR .

JOURNAL OF BACTERIOLOGY, 1994, 176 (15) :4534-4542

[2] PRODUCTION OF HEMOLYSIN AND OTHER EXTRACELLULAR ENZYMES BY CLINICAL ISOLATES OF PSEUDOMONAS-PSEUDOMALLEI [J].

ASHDOWN, LR ;

KOEHLER, JM .

JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (10) :2331-2334

[3] A second type III secretion system in Burkholderia pseudomallei:: who is the real culprit? [J].

Attree, O ;

Attree, I .

MICROBIOLOGY-SGM, 2001, 147 :3197-3199

[4] How to survive in the host:: the Yersinia lesson [J].

Bleves, S ;

Cornelis, GR .

MICROBES AND INFECTION, 2000, 2 (12) :1451-1460

[5] Characterization of Burkholderia pseudomallei and Burkholderia pseudomallei-like strains [J].

Brett, PJ ;

Deshazer, D ;

Woods, DE .

EPIDEMIOLOGY AND INFECTION, 1997, 118 (02) :137-148

[6] Identification of the acid phosphatase (acpA) gene homologues in pathogenic and nonpathogenic Burkholderia spp. facilitates TnphoA mutagenesis [J].

Burtnick, MN ;

Bolton, AJ ;

Brett, PJ ;

Watanabe, D ;

Woods, DE .

MICROBIOLOGY-UK, 2001, 147 :111-120

[7] Endemic melioidosis in tropical northern Australia: A 10-year prospective study and review of the literature [J].

Currie, BJ ;

Fisher, DA ;

Howard, DM ;

Burrow, JNC ;

Lo, D ;

Selva-Nayagam, S ;

Anstey, NM ;

Huffam, SE ;

Snelling, PL ;

Marks, PJ ;

Stephens, DP ;

Lum, GD ;

Jacups, SP ;

Krause, VL .

CLINICAL INFECTIOUS DISEASES, 2000, 31 (04) :981-986

[8] The type II O-antigenic polysaccharide moiety of Burkholderia pseudomallei lipopolysaccharide is required for serum resistance and virulence [J].

DeShazer, D ;

Brett, PJ ;

Woods, DE .

MOLECULAR MICROBIOLOGY, 1998, 30 (05) :1081-1100

[9] Salmonella interactions with host cells:: in vitro to in vivo [J].

Finlay, BB ;

Brumell, JH .

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 2000, 355 (1397) :623-631

[10] DIFFERENTIAL PLASMID RESCUE FROM TRANSGENIC MOUSE DNAS INTO ESCHERICHIA-COLI METHYLATION-RESTRICTION MUTANTS [J].

GRANT, SGN ;

JESSEE, J ;

BLOOM, FR ;

HANAHAN, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (12) :4645-4649

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