In vitro kinetics of allergen- and microbe-induced IL-4 and IFN-γ mRNA expression in PBMC of pollen-allergic patients

被引:9
作者
Laaksonen, K
Waris, M
Mäkelä, MJ
Terho, EO
Savolainen, J
机构
[1] Univ Turku, Dept Pulm Dis & Clin Allergol, FIN-20520 Turku, Finland
[2] Univ Helsinki, Cent Hosp, Skin & Allergy Hosp, Dept Allergol, Helsinki, Finland
关键词
allergen; kinetics; tetanus toxin; tuberculin; IL-4; IFN-gamma;
D O I
10.1034/j.1398-9995.2003.23653.x
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
Background: According to a hypothesis allergens induce Th2 responses in allergic patients, and microbes induce Th1 responses. We studied the kinetics of in vitro allergen-, tuberculin (PPD)- and tetanus toxin (TT)-induced IFN-gamma and IL-4 mRNA expression in peripheral blood mononuclear cell (PBMC) cultures of pollen-allergic patients and healthy controls. Methods: PBMC of 10 birch or timothy pollen-allergic patients and of 13 healthy controls were stimulated in vitro with allergen (birch or timothy), PPD or TT. Pellets and supernatants were collected at 24, 48, 72 and 96 h after stimulation. IFN-gamma and IL-4 production was measured by enzyme linked immunosorbent assay and mRNA expression using RT-PCR and time-resolved fluorometry. Results: Allergen induced IFN-gamma production and mRNA expression in PBMC more in allergic patients than in healthy controls. Also allergen induced IL-4 mRNA expression more in allergic patients than in healthy controls. PPD induced IFN-gamma mRNA expression both in allergic patients and healthy controls, whereas IFN-gamma production was induced only in healthy controls and IL-4 was not induced at all. TT induced IFN-gamma mRNA expression in both groups, IFN-gamma production in allergic patients, and IL-4 mRNA expression in both allergic patients and healthy controls. Conclusions: In vitro stimulation with allergen induced both IFN-gamma and IL-4 mRNA expression of PBMC in allergic patients. These observations challenge the clearcut division of microbe-specific Th1 and allergen-specific Th2 responses in peripheral blood.
引用
收藏
页码:62 / 66
页数:5
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