Sex determination by simultaneous amplification of equine SRY and amelogenin genes

被引：59

作者：

Hasegawa, T ^{[1
]}

Sato, F

Ishida, N

Fukushima, Y

Mukoyama, H

机构：

[1] Japan Racing Assoc, Equine Res Inst, Mol & Cellular Biol Lab, Utsunomiya, Tochigi 3200856, Japan

[2] Tochigi Prefecture Police Headquarters, Forens Sci Lab, Utsunomiya, Tochigi 3200027, Japan

[3] Tokyo Univ Agr, Fac Agr, Dept Agr, Atsugi, Kanagawa 2430034, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2000年 / 62卷 / 10期

关键词：

amelogenin; equine sex-determination; SRY;

D O I：

10.1292/jvms.62.1109

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A quick method for sex determination of horses was developed. Simultaneous amplification of the equine sex-determining region of the Y chromosome gene (SRY) and amelogenin gene (AMEL) accomplished the determination of the presence of both the Y chromosome and SRY gene. In agarose gel electrophoresis, a normal stallion showed 1 SRY band and 3 AMEL (AMELX, AMELY, and AMELX/AMELY heteroduplex) bands, and a normal mare showed a single AMELX band. In XY-mares, 3 AMEL bands were detected as in a normal stallion, but no SRY band. The present method enables a quick diagnosis for XY-mare prior to cytogenetic analysis.

引用

页码：1109 / 1110

页数：2

共 9 条

[1]

Abe S, 1999, EQUINE VET J, V31, P336, DOI 10.1111/j.2042-3306.1999.tb03813.x

[2]

Fukushima Yasutoshi, 1999, Animal Science Journal, V70, pJ6

[3] Linear SRY transcript in equine testis [J].