Replication properties of dUTPase-deficient mutants of caprine and ovine lentiviruses

被引：77

作者：

Turelli, P

Petursson, G

Guiguen, F

Mornex, JF

Vigne, R

Querat, G

机构：

[1] INSERM,U372,F-13276 MARSEILLE 9,FRANCE

[2] ECOLE VET LYON,INRA,F-69280 MARCY LETOILE,FRANCE

来源：

JOURNAL OF VIROLOGY | 1996年 / 70卷 / 02期

关键词：

D O I：

10.1128/JVI.70.2.1213-1217.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The virion-associated dUTPase activities of caprine arthritis-encephalitis virus (CAEV) and visna virus were determined by using an assay which measures the actual ability of the dUTPase to prevent the dUTP misincorporations into cDNA during reverse transcription. We showed that the CAEV molecular clone from the Cork isolate was dUTPase defective as a result of a single amino acid substitution. Using this point mutant and deletion mutants of CAEV as well as a deletion mutant of visna virus, we demonstrated that dUTPase-deficient viruses replicate similarly to wild-type viruses in dividing cells but show delayed replication in nondividing primary macrophages.

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页码：1213 / 1217

页数：5

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