Electroporation-mediated transformation of Arcanobacterium (Actinomyces) pyogenes

被引：16

作者：

Jost, BH ^{[1
]}

Billington, SJ ^{[1
]}

Songer, JG ^{[1
]}

机构：

[1] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA

来源：

PLASMID | 1997年 / 38卷 / 02期

关键词：

D O I：

10.1006/plas.1997.1299

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Plasmids derived from pNG2 or RSF1010 were introduced into strains of Arcanobacterium (Actinomyces) pyogenes by electroporation. Electroporation conditions were varied systematically to give a maximum electroporation frequency of 3.7 x 10(5) CFU/mu g DNA at 1.5 kV/cm and 246 Omega, resulting in a rime constant of approximately 10 ms. The A. pyogenes transformants expressed plasmid-encoded resistance to chloramphenicol, erythromycin, kanamycin, and streptomycin. The source of incoming DNA affected the growth rate of transformants, but not the electroporation efficiency. This is the first report of genetic transformation of the veterinary pathogen A. pyogenes. (C) 1997 Academic Press.

引用

页码：135 / 140

页数：6

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