A conformational flexibility scale for amino acids in peptides

被引：178

作者：

Huang, F

Nau, WM

机构：

[1] Int Univ Bremen, Sch Sci & Engn, D-28759 Bremen, Germany

[2] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2003年 / 42卷 / 20期

关键词：

amino acids; fluorescence; peptides; protein folding; secondary structure;

D O I：

10.1002/anie.200250684

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The end-to-end collision frequency of short polypeptides labeled with a fluorescent probe (DBO) at one end and an efficient contact quencher (Trp) at the other end (see scheme) provides an absolute measure of the time scale of conformational changes in short structureless peptides as a function of the amino acid type. A general correlation with secondary structure propensity applies, with the most flexible amino acids being most abundant in β turns and the most rigid ones in β sheets.

引用

收藏

页码：2269 / 2272

页数：4

相关论文

共 39 条

[11] FLEXIBILITY AND RIGIDITY, REQUIREMENTS FOR THE FUNCTION OF PROTEINS AND PROTEIN PIGMENT COMPLEXES [J].

HUBER, R .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1987, 15 (06) :1009-1020

[12] A fluorescence-based method for direct measurement of submicrosecond intramolecular contact formation in biopolymers: An exploratory study with polypeptides [J].

Hudgins, RR ;

Huang, F ;

Gramlich, G ;

Nau, WM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (04) :556-564

[13] A REVISED SET OF POTENTIALS FOR BETA-TURN FORMATION IN PROTEINS [J].

HUTCHINSON, EG ;

THORNTON, JM .

PROTEIN SCIENCE, 1994, 3 (12) :2207-2216

[14] PREDICTION OF CHAIN FLEXIBILITY IN PROTEINS - A TOOL FOR THE SELECTION OF PEPTIDE ANTIGENS [J].

KARPLUS, PA ;

SCHULZ, GE .

NATURWISSENSCHAFTEN, 1985, 72 (04) :212-213

[15]

Koca J, 1998, PROG BIOPHYS MOL BIO, V70, P137, DOI 10.1016/S0079-6107(98)00029-7

[16] Structure-based conformational preferences of amino acids [J].

Koehl, P ;

Levitt, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (22) :12524-12529

[17] Effects of chain stiffness on the dynamics of loop formation in polypeptides. Appendix: Testing a 1-dimensional diffusion model for peptide dynamics [J].

Lapidus, LJ ;

Steinbach, PJ ;

Eaton, WA ;

Szabo, A ;

Hofrichter, J .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (44) :11628-11640

[18] CONFORMATIONAL PREFERENCES OF AMINO-ACIDS IN GLOBULAR PROTEINS [J].

LEVITT, M .

BIOCHEMISTRY, 1978, 17 (20) :4277-4284

[19]

Mikhailov DV, 1999, BIOPOLYMERS, V49, P373, DOI 10.1002/(SICI)1097-0282(19990415)49:5<373::AID-BIP4>3.0.CO

[20]

2-V

← 1 2 3 4 →