A soluble, magnesium-independent prenyltransferase catalyzes reverse and regular C-prenylations and O-prenylations of aromatic substrates

被引：50

作者：

Haagen, Yvonne

Unsoeld, Inge

Westrich, Lucia

Gust, Bertolt

Richard, Stephane B.

Noel, Joseph P.

Heide, Lutz

机构：

[1] Univ Tubingen, Inst Pharmazeut, D-72076 Tubingen, Germany

[2] Salk Inst Biol Studies, Jack Skirball Chem Biol & Proteom Lab, La Jolla, CA 92037 USA

来源：

FEBS LETTERS | 2007年 / 581卷 / 16期

关键词：

prenyltransferase; reverse prenylation; furanonaphthoquinone; Streptomyces;

D O I：

10.1016/j.febslet.2007.05.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fnq26 from Streptomyces cinnamonensis DSM 1042 is a new member of the recently identified CloQ/Orf2 class of prenyltransferases. The enzyme was overexpressed in E. coli and purified to apparent homogeneity, resulting in a soluble, monomeric protein of 33.2 kDa. The catalytic activity of Fnq26 is independent of the presence of Mg2+ or other divalent metal ions. With flaviolin (2,5,7-trihydroxy-1,4-naphthoquinone) as substrate, Fnq26 catalyzes the formation of a carbon-carbon-bond between C-3 (rather than C-1) of geranyl diphosphate and C-3 of flaviolin, i.e. an unusual "reverse" prenylation. With 1,3-dihydroxynaphthalene and 4-hydroxybenzoate as substrates Fnq26 catalyzes O-prenylations. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：2889 / 2893

页数：5

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