Molecular cloning of novel cellulase genes cel9A and cel12A from Bacillus licheniformis GXN151 and Synergism of their encoded polypeptides

被引：21

作者：

Liu, YS ^{[1
]}

Zhang, J

Liu, Q

Zhang, CG

Ma, QS

机构：

[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China

[2] Chinese Acad Sci, Shenyang Inst Appl Ecol, Shenyang, Peoples R China

[3] Lanzhou Univ, Sch Life Sci, Lanzhou, Peoples R China

来源：

CURRENT MICROBIOLOGY | 2004年 / 49卷 / 04期

关键词：

D O I：

10.1007/s00284-004-4291-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A thermophilic bacterial strain GXN151 which could degrade Avicel efficiently was isolated and identified as Bacillus licheniformis. A genomic library of GXN151 was constructed and two novel endoglucanase genes designated cel9A and cel12A were isolated by screening the library on carboxylmethyl cellulase indicator plates. The analysis of amino acid sequences deduced from the genes indicated that Cel9A consisted of a catalytic domain belonging to glycosyl hydrolase family 9, a linker domain, and a carbohydrate binding module family 3 from N-terminal to C-terminal; Cel12A had only one catalytic domain belonging to glycosyl hydrolase family 12. The combinations of Cel9A and Cel12A produced by the recombinant E. coli exhibited synergistic action against substrates of carboxylmethyl cellulose as well as Avicel.

引用

页码：234 / 238

页数：5

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