Colloidal Nanogold-Based Immunochromatographic Strip Test for the Detection of Digoxin Toxicity

被引：52

作者：

Omidfar, Kobra ^{[1
]}

Kia, Solmaz ^{[2
]}

Kashanian, Soheila ^{[3
,4
]}

Paknejad, Malihe ^{[5
]}

Besharatie, Afsaneh ^{[1
]}

Kashanian, Susan ^{[1
]}

Larijani, Bagher ^{[6
]}

机构：

[1] Univ Tehran Med Sci, Biochem Lab, Endocrinol & Metab Res Ctr, Tehran, Iran

[2] Razi Univ, Sch Sci, Dept Biol, Kermanshah, Iran

[3] Razi Univ, Fac Sci, Dept Chem, SBRC, Kermanshah, Iran

[4] Razi Univ, Fac Sci, NNRC, Kermanshah, Iran

[5] Univ Tehran Med Sci, Dept Biochem, Sch Med, Tehran, Iran

[6] Univ Tehran Med Sci, Endocrinol & Metab Res Ctr, Tehran, Iran

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 160卷 / 03期

关键词：

Digoxin; Immunochromatography; Colloidal nanogold; Monoclonal antibody; LINKED CHEMILUMINESCENT IMMUNOSORBENT; OF-CARE TEST; ASSAY; IMMUNOASSAY; RESIDUES; INTERFERENCE; GOLD;

D O I：

10.1007/s12010-009-8535-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Digoxin is widely used as a cardiac glycoside drug in the treatment of various heart conditions. Because it is a toxic drug, it should be regularly monitored in the serum of patients under treatment. In this study, colloidal nanogold is synthesized and the preparation of nanogold-labeled monoclonal antibody probe to digoxin is described under optimal conditions. In addition, an immunochromatographic (IC) method for digoxin analysis employing nanogold-labeled probe is developed. With this technique, it requires only 5 min to complete the quantitative detection of digoxin. The detection time is decreased 20-30 times in comparison to radioimmunoassay (RIA). The sensitivity to digoxin was about 2 ng/ml by naked eye, which is within the therapeutic and toxic ranges of digoxin. The results of serum samples obtained by IC strip were in agreement with those obtained by RIA. The IC strip was sufficiently sensitive and accurate to be used for the rapid detection of digoxin in serum samples.

引用

页码：843 / 855

页数：13

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