Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2β

被引：22

作者：

Burbelo, Peter D. ^{[1
]}

Hirai, Hiroki ^{[2
]}

Issa, Alexandra T. ^{[1
]}

Kingman, Albert

Lernmark, Ake ^{[3
]}

Ivarsson, Sten-A. ^{[3
]}

Notkins, Abner L. ^{[2
]}

Iadarola, Michael J. ^{[1
]}

机构：

[1] Natl Inst Dent & Craniofacial Res, Neurobiol & Pain Therapeut Sect, Lab Sensory Biol, NIH, Bethesda, MD 20892 USA

[2] Natl Inst Dent & Craniofacial Res, Expt Med Sect, Oral Infect & Immun Branch, NIH, Bethesda, MD USA

[3] Malmo Univ Hosp, Dept Paediat, Malmo, Sweden

来源：

DIABETES CARE | 2010年 / 33卷 / 04期

基金：

美国国家卫生研究院;

关键词：

AUTOANTIGENS; DISEASE;

D O I：

10.2337/dc09-1938

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

引用

页码：754 / 756

页数：3

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