Elicitation of suspension-cultured tomato cells triggers the formation of phosphatidic acid and diacylglycerol pyrophosphate

被引:175
作者
van der Luit, AH
Piatti, T
van Doorn, A
Musgrave, A
Felix, G
Boller, T
Munnik, T
机构
[1] Univ Amsterdam, Dept Plant Physiol, Swammerdam Inst Life Sci, NL-1098 SM Amsterdam, Netherlands
[2] Friedrich Miescher Inst, CH-4002 Basel, Switzerland
关键词
D O I
10.1104/pp.123.4.1507
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Phosphatidic acid (PA) and its phosphorylated derivative diacylglycerol pyrophosphate (DGPP) are lipid molecules that have been implicated in plant cell signaling. In this study we report the rapid but transient accumulation of PA and DGPP in suspension-cultured tomato (Lycopersicon esculentum) cells treated with the general elicitors, N,N',N ",N'''-tetraacetylchitotetraose, xylanase, and the flagellin-derived peptide flg22. To determine whether PA originated from the activation of phospholipase D or from the phosphorylation of diacylglycerol (DAG) by DAG kinase, a strategy involving differential radiolabeling with [P-32]orthophosphate was used. DAG kinase was found to be the dominant producer of PA that was subsequently metabolized to DGPP. A minor but significant role for phospholipase D could only be detected when xylanase was used as elicitor. Since Ph formation was correlated with the high turnover of polyphosphoinositides, we hypothesize that elicitor treatment activates phospholipase C to produce DAG, which in turn acts as substrate for DAG kinase. The potential roles of PA and DGPP in plant defense signaling are discussed.
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收藏
页码:1507 / 1515
页数:9
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