Role of connexin-43 hemichannels in the pathogenesis of Yersinia enterocolitica

Connexin (Cx) channels are sites of cytoplasmic communication between contacting cells Evidence indicates that the opening of hemichannel occurs under both physiological and pathological conditions In this paper. the involvement of Cx-43 hemichannels is demonstrated in the pathogenesis of Yersinia. Parental Hela cells and transfected HeLa cells stably expressing Cx-43 (HCx43) were infected with Yersinia enterocolitica, and bacterial uptake was measured by the colony-forming, unit method Bacterial uptake was hi.-her in HCx43 cells than in parental cells and was inhibited by the Cx channel blocker, 18-alpha-glycyrrhetinic acid (AGA). The inhibitory effect of AGA was more pronounced on the Y enterocolitica uptake by HCx43 cells than by parental cells The ability of HCx43 cells to incorporate the permeable fluorescent tracer Lucifer Yellow (LY) was assessed Dye incorporation was inhibited by AGA, whereas Y enterocalitica infection of HCx43 cells increased LY incorporation. Western blotting analysis demonstrated that Y. enterocolitica infection of IACx43 cells induced tyrosine phosphorylation of Cx-43, thus supporting a critical role for Cx-43 in the strategies exploited by bacterial pathogens to invade nonphagocytic cells (C) 2008 Elsevier Ltd. All rights reserved.