Tissue distribution and molecular characterization of chicken isolates of Toxoplasma gondii from Peru

被引：39

作者：

Dubey, JP ^{[1
]}

Levy, MZ ^{[1
]}

Sreekumar, C ^{[1
]}

Kwok, OCH ^{[1
]}

Shen, SK ^{[1
]}

Dahl, E ^{[1
]}

Thulliez, P ^{[1
]}

Lehmann, T ^{[1
]}

机构：

[1] USDA ARS, Beltsville Agr Res Ctr, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

来源：

JOURNAL OF PARASITOLOGY | 2004年 / 90卷 / 05期

关键词：

D O I：

10.1645/GE-329R

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of T. gondii oocysts in the soil because chickens feed from the ground. The prevalence of T. gondii antibodies in sera of 50 free-range chickens (Gallus domesticus) from Peru was 26% on the basis of the modified agglutination test (MAT). Hearts, pectoral muscles, and brains of seropositive (MAT greater than or equal to1:5) chickens were bioassayed individually in mice. Tissues from the remaining 37 seronegative chickens were pooled and fed to 2 T. gondii-free cats. Feces of cats were examined for oocysts; they did not shed oocysts. Toxoplasma gondii was isolated from the hearts of 10 seropositive chickens but not from their brains and pectoral muscles. Genotyping of these isolates using the SAG2 locus indicated that 7 isolates were type I and 3 were type III. Six of the 7 type-I isolates were avirulent for mice, which was unusual because type-I isolates are considered virulent for mice. The T. gondii isolates were from chickens from different properties that were at least 200 m apart. Thus, each isolate is likely to be different. This is the first report of isolation of T. gondii from chickens from Peru.

引用

页码：1015 / 1018

页数：4

共 31 条

[1] Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings [J].