GISH confirmation of somatic hybrids between Solanum melongena and S-torvum: assessment of resistance to both fungal and bacterial wilts

被引:25
作者
Collonnier, C
Fock, I
Mariska, I
Servaes, A
Vedel, F
Siljak-Yakovlev, S
Souvannavong, V
Sihachakr, D [1 ]
机构
[1] Ctr Univ Paris Sud, CNRS, Syst Evolut, Lab Ecol Systemat & Evolut,UMR 8079, Bat 360, F-91405 Orsay, France
[2] Univ Reunion, Genet Mol & Evolut, Lab Biol & Physiol Vegetales Genet Mol & Evolut, F-97715 St Denis, France
[3] Res Inst Food Crops Biotechnol, Bogor 16610, Indonesia
[4] Univ Paris 11, CNRS, Grp Endotoxines, UMR 8619, F-91405 Orsay, France
关键词
GISH; in vitro tests; protoplast electrofusion; Ralstonia solanacearum; Solanum melongena; Solanum torvum; Verticillium dahliae;
D O I
10.1016/S0981-9428(03)00054-8
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Interspecific somatic hybrids between Solanum melongena L. (2n = 2x = 24) and two accessions of Solanum torvum Sw. (2n = 2x = 24) were produced in view of transferring resistance to two soil-born pathogens, Ralstonia solanacearum and Verticillium dahliae, from the wild species into the cultivated eggplant. All somatic hybrids were phenotypically homogenous and intermediate between the parents. Their hybrid nature was confirmed by analysis of isozymes and RAPDs. They showed reduced pollen viability, and all but one possessed the chloroplasts from either one or the other parent. As S. melongena and S. torvum chromosomes were morphologically indistinguishable, genomic in situ hybridisation (GISH) was applied to recognise the chromosomes from each parent in the hybrids. As expected, the selected tetraploid plants contained one complete set of chromosomes from each fusion partner. On spread preparations, the two parental genomes were not spatially separated at any time of the cell cycle. Translocation or recombinant chromosomes could not be demonstrated in the mitotic metaphase. Tests for resistance performed in vitro by using suspensions of two strains of R. solanacearum (race 1 and 3) and filtrate of culture medium of one strain of V dahliae, revealed that S. melongena was susceptible, whereas both accessions of S. torvum had high levels of resistance. Except for two hybrid clones, which were found susceptible to race 3, as was S. melongena, all somatic hybrids tested showed good levels of bacterial and fungal resistance, either intermediate or as high as that of the wild parent. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
引用
收藏
页码:459 / 470
页数:12
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