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Cloning and nucleotide sequence of the DNA gyrase gyrA gene from Serratia marcescens and characterization of mutations in gyrA of quinolone-resistant clinical isolates

被引：23
作者：
Kim, JH
Cho, EH
Kim, KS
Kim, HY
Kim, YM [1 ]
机构：
[1] Yonsei Univ, Dept Biol, Mol Microbiol Lab, Seoul 120749, South Korea
[2] Chosun Univ, Dept Sci Educ, Kwangju 501759, South Korea
[3] C&C Res Labs, Dept Pharmacol & Mol Biol, Kyunggi Do 445970, South Korea
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1998年 / 42卷 / 01期
关键词：
D O I：
10.1128/AAC.42.1.190
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The sequence of the DNA gyrase gyrA gene of Serratia marcescens ATCC 14756 was determined, An open reading frame of 2,640 nucleotides coding for a polypeptide with a calculated molecular mass of 97,460 was found, and its sequence complemented the sequence of an Escherichia coli gyrA temperature-sensitive mutation. Analysis of the PCR products of the quinolone resistance-determining regions of gyrA genes from six quinolone-resistant clinical isolates revealed a single amino acid substitution, Ser-83 to Arg or Asp-87 to Tyr, in all six mutants, suggesting that a mutational alteration in gyrA is a common mechanism of quinolone resistance in S. marcescens.
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页码：190 / 193
页数：4
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