Capillary Liquid Chromatography at Ultrahigh Pressures

被引：84

作者：

Jorgenson, James W. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 3 | 2010年 / 3卷

关键词：

UHPLC; high-resolution liquid chromatography; 1-mu m particles; OPEN-TUBULAR COLUMNS; POROUS SILICA LAYERS; STATIONARY PHASES; ELEVATED-TEMPERATURE; SOLUTE RETENTION; INJECTION VALVE; INNER DIAMETERS; PERFORMANCE; PARTICLES; SEPARATION;

D O I：

10.1146/annurev.anchem.1.031207.113014

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Ultrahigh-pressure liquid chromatography (UHPLC) is a method of liquid chromatography utilizing sub-2-mu m particles packed into capillary columns 25 to 100 cm long. Columns of this length packed with particles this fine require operation with pressures from 1,000 to 7,000 bar (15,000 to 100,000 psi). The advantages of this technique are high separation powers (theoretical plate counts from 100,000 to 300,000) and run times from a few minutes (isocratic) to a few hours (long gradients). This review discusses the background and theoretical basis of UHPLC, practical aspects of UHPLC hardware, examples of separations, future areas for research in UHPLC, and techniques that are both competitive with and complementary to UHPLC.

引用

页码：129 / 150

页数：22

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