Cellular impedance biosensors for drug screening and toxin detection

被引:131
作者
Asphahani, Fareid [1 ]
Zhang, Miqin [1 ]
机构
[1] Univ Washington, Dept Mat Sci & Engn, Seattle, WA 98195 USA
关键词
D O I
10.1039/b704513a
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Cell-based impedance biosensing is an emerging technology that can be used to non-invasively and instantaneously detect and analyze cell responses to chemical and biological agents. This article highlights the fabrication and measurement technologies of cell impedance sensors, and their application in toxin detection and anti-cancer drug screening. We start with an introduction that describes the capability and advantages of cell-based sensors over conventional sensing technology, followed by a discussion of the influence of cell adhesion, spreading and viability during cell patterning on the subsequent impedance measurements and sensing applications. We then present an electronic circuit that models the cell-electrode system, by which the cellular changes can be detected in terms of impedance changes of the circuit. Finally, we discuss the current status on using cell impedance sensors for toxin detection and anti-cancer drug screening.
引用
收藏
页码:835 / 841
页数:7
相关论文
共 68 条
[1]   Bioelectrical impedance assay to monitor changes in cell shape during apoptosis [J].
Arndt, S ;
Seebach, J ;
Psathaki, K ;
Galla, HJ ;
Wegener, J .
BIOSENSORS & BIOELECTRONICS, 2004, 19 (06) :583-594
[2]   Engineering mammalian cells for solid-state sensor applications [J].
Bloom, FR ;
Price, P ;
Lao, GF ;
Xia, JL ;
Crowe, JH ;
Battista, JR ;
Helm, RF ;
Slaughter, S ;
Potts, M .
BIOSENSORS & BIOELECTRONICS, 2001, 16 (7-8) :603-608
[3]  
BORKHOLDER DA, 1998, THESIS STANFORD U PA
[4]   Whole cell biosensors [J].
Bousse, L .
SENSORS AND ACTUATORS B-CHEMICAL, 1996, 34 (1-3) :270-275
[5]   Micropatterned surfaces for control of cell shape, position, and function [J].
Chen, CS ;
Mrksich, M ;
Huang, S ;
Whitesides, GM ;
Ingber, DE .
BIOTECHNOLOGY PROGRESS, 1998, 14 (03) :356-363
[6]   Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures [J].
Ehret, R ;
Baumann, W ;
Brischwein, M ;
Schwinde, A ;
Stegbauer, K ;
Wolf, B .
BIOSENSORS & BIOELECTRONICS, 1997, 12 (01) :29-41
[7]  
Folch A, 2000, J BIOMED MATER RES, V52, P346, DOI 10.1002/1097-4636(200011)52:2<346::AID-JBM14>3.0.CO
[8]  
2-H
[9]   Cellular micropatterns on biocompatible materials [J].
Folch, A ;
Toner, M .
BIOTECHNOLOGY PROGRESS, 1998, 14 (03) :388-392
[10]   Microengineering of cellular interactions [J].
Folch, A ;
Toner, M .
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING, 2000, 2 :227-+