Evaluation of a fluorescence-based PCR method for identification of serogroup A meningococci

被引：10

作者：

Diggle, MA

Smith, K

Girvan, EK

Clarke, SC

机构：

[1] Stobhill Gen Hosp, Dept Microbiol, N Glasgow Univ Hosp NHS Trust, Scottish Meningococcus & Pneumococcus Reference L, Glasgow G21 3UW, Lanark, Scotland

[2] Univ Glasgow, Fac Biomed & Life Sci, Glasgow, Lanark, Scotland

[3] Wishaw Gen Hosp, Dept Microbiol, Wishaw, Scotland

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 04期

关键词：

D O I：

10.1128/JCM.41.4.1766-1768.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.

引用

页码：1766 / 1768

页数：3

共 8 条

[1] EPIDEMIC SPREAD AND ANTIGENIC VARIABILITY OF NEISSERIA-MENINGITIDIS
ACHTMAN, M
[J]. TRENDS IN MICROBIOLOGY, 1995, 3 (05) : 186 - 192
[2] Clarke SC, 2001, BRIT J BIOMED SCI, V58, P230
[3] Molecular divergence of the sia locus in different serogroups of Neisseria meningitidis expressing polysialic acid capsules
Claus, H
Vogel, U
Mühlenhoff, M
Gerardy-Schahn, R
Frosch, M
[J]. MOLECULAR AND GENERAL GENETICS, 1997, 257 (01): : 28 - 34
[4] Is group C meningococcal disease increasing in Europe? A report of surveillance of meningococcal infection in Europe 1993-6
Connolly, M
Noah, N
[J]. EPIDEMIOLOGY AND INFECTION, 1999, 122 (01) : 41 - 49
[5] Automation of fluorescence-based PCR for confirmation of meningococcal disease
Diggle, MA
Edwards, GFS
Clarke, SC
[J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (12) : 4518 - 4519
[6] Timely detection of meningococcal meningitis epidemics in Africa
Lewis, R
Nathan, N
Diarra, L
Belanger, F
Paquet, C
[J]. LANCET, 2001, 358 (9278) : 287 - 293
[7] The changing epidemiology of meningococcal disease in the United States 1992-1996
Rosenstein, NE
Perkins, BA
Stephens, DS
Lefkowitz, L
Cartter, ML
Danila, R
Cieslak, P
Shutt, KA
Popovic, T
Schuchat, A
Harrison, LH
Reingold, AL
[J]. JOURNAL OF INFECTIOUS DISEASES, 1999, 180 (06) : 1894 - 1901
[8] Characterization of the gene cassette required for biosynthesis of the (α1→6)-linked N-acetyl-D-mannosamine-1-phosphate capsule of serogroup A Neisseria meningitidis
Swartley, JS
Liu, LJ
Miller, YK
Martin, LE
Edupuganti, S
Stephens, DS
[J]. JOURNAL OF BACTERIOLOGY, 1998, 180 (06) : 1533 - 1539

← 1 →