Multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors

被引:132
作者
Yang, Minghui [2 ]
Li, He [2 ]
Javadi, Alireza [3 ]
Gong, Shaoqin [1 ,2 ,3 ]
机构
[1] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Mech Engn, Milwaukee, WI 53211 USA
[3] Univ Wisconsin, Dept Mat, Milwaukee, WI 53211 USA
基金
美国国家科学基金会;
关键词
Enzyme; Electrochemistry; Mesoporous silica nanoparticle; Immunosensor; REAGENTLESS AMPEROMETRIC IMMUNOSENSOR; RESPONSIVE CONTROLLED-RELEASE; CANCER BIOMARKER PROTEINS; HORSERADISH-PEROXIDASE; DELIVERY-SYSTEM; DRUG-DELIVERY; AMPLIFICATION; IMMUNOASSAY; NANOSPHERE; ELECTRODES;
D O I
10.1016/j.biomaterials.2010.01.033
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using MSN-TH-HRP-Ab(2) as labels for human IgG detection was about 100 times higher than that using either MSN-TH-Ab(2) or MSN-HRP-Ab(2) as labels, indicating the high catalytic efficiency of HRP in the presence of mediator TH toward H(2)O(2). The immunosensor using MSN-TH-Ab(2) as labels exhibited a high sensitivity and showed a linear response within the range of 0.01-10 ng/mL human IgG. It also showed good reproducibility and selectivity and acceptable stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3281 / 3286
页数:6
相关论文
共 30 条
  • [11] Disposable electrochemical immunosensor diagnosis device based on nanoparticle probe and immunochromatographic strip
    Liu, Guodong
    Lin, Ying-Ying
    Wang, Jun
    Wu, Hong
    Wai, Chien M.
    Lin, Yuehe
    [J]. ANALYTICAL CHEMISTRY, 2007, 79 (20) : 7644 - 7653
  • [12] PLANT PEROXIDASE
    MAEHLY, AC
    [J]. METHODS IN ENZYMOLOGY, 1955, 2 : 801 - 813
  • [13] Ultrasensitive Immunosensor for Cancer Biomarker Proteins Using Gold Nanoparticle Film Electrodes and Multienzyme-Particle Amplification
    Mani, Vigneshwaran
    Chikkaveeraiah, Bhaskara V.
    Patel, Vyomesh
    Gutkind, J. Silvio
    Rusling, James F.
    [J]. ACS NANO, 2009, 3 (03) : 585 - 594
  • [14] Gatekeeping layer effect: A poly(lactic acid)-coated mesoporous silica nanosphere-based fluorescence probe for detection of amino-containing neurotransmitters
    Radu, DR
    Lai, CY
    Wiench, JW
    Pruski, M
    Lin, VSY
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (06) : 1640 - 1641
  • [15] An array immunosensor for simultaneous detection of clinical analytes
    Rowe, CA
    Scruggs, SB
    Feldstein, MJ
    Golden, JP
    Ligler, FS
    [J]. ANALYTICAL CHEMISTRY, 1999, 71 (02) : 433 - 439
  • [16] Thionine covalently tethered to multilayer horseradish peroxidase in a self-assembled monolayer as an electron-transfer mediator
    Ruan, CM
    Yang, F
    Lei, CH
    Deng, JQ
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (09) : 1721 - 1725
  • [17] Designing nanomaterial-enhanced electrochemical immunosensors for cancer biomarker proteins
    Rusling, James F.
    Sotzing, Gregory
    Papadimitrakopoulosa, Fotios
    [J]. BIOELECTROCHEMISTRY, 2009, 76 (1-2) : 189 - 194
  • [18] Detection of Salmonella typhimurium using an electrochemical immunosensor
    Salam, Faridah
    Tothill, Ibtisam E.
    [J]. BIOSENSORS & BIOELECTRONICS, 2009, 24 (08) : 2630 - 2636
  • [19] Effect of surface functionalization of MCM-41-type mesoporous silica nanoparticleson the endocytosis by human cancer cells
    Slowing, Igor
    Trewyn, Brian G.
    Lin, Victor S. -Y.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (46) : 14792 - 14793
  • [20] Mesoporous silica nanoparticles for intracellular delivery of membrane-impermeable proteins
    Slowing, Igor I.
    Trewyn, Brian G.
    Lin, Victor S. -Y.
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (28) : 8845 - 8849