共 30 条
Multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors
被引:132
作者:
Yang, Minghui
[2
]
Li, He
[2
]
Javadi, Alireza
[3
]
Gong, Shaoqin
[1
,2
,3
]
机构:
[1] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA
[2] Univ Wisconsin, Dept Mech Engn, Milwaukee, WI 53211 USA
[3] Univ Wisconsin, Dept Mat, Milwaukee, WI 53211 USA
基金:
美国国家科学基金会;
关键词:
Enzyme;
Electrochemistry;
Mesoporous silica nanoparticle;
Immunosensor;
REAGENTLESS AMPEROMETRIC IMMUNOSENSOR;
RESPONSIVE CONTROLLED-RELEASE;
CANCER BIOMARKER PROTEINS;
HORSERADISH-PEROXIDASE;
DELIVERY-SYSTEM;
DRUG-DELIVERY;
AMPLIFICATION;
IMMUNOASSAY;
NANOSPHERE;
ELECTRODES;
D O I:
10.1016/j.biomaterials.2010.01.033
中图分类号:
R318 [生物医学工程];
学科分类号:
0831 ;
摘要:
Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using MSN-TH-HRP-Ab(2) as labels for human IgG detection was about 100 times higher than that using either MSN-TH-Ab(2) or MSN-HRP-Ab(2) as labels, indicating the high catalytic efficiency of HRP in the presence of mediator TH toward H(2)O(2). The immunosensor using MSN-TH-Ab(2) as labels exhibited a high sensitivity and showed a linear response within the range of 0.01-10 ng/mL human IgG. It also showed good reproducibility and selectivity and acceptable stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules. (C) 2010 Elsevier Ltd. All rights reserved.
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页码:3281 / 3286
页数:6
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