In vivo expression technology strategies:: valuable tools for biotechnology

被引:39
作者
Rainey, PB [1 ]
Preston, GM [1 ]
机构
[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1016/S0958-1669(00)00132-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Whole genome sequences have shown that bacteria possess a significant number of genes that have no known function. It is probable that many of these are required for survival in environments other than the agar plate. In vivo selection strategies provide a means of obtaining genes active in complex natural environments. Direct access to these genes is essential for understanding ecological performance and provides novel opportunities for biotechnology.
引用
收藏
页码:440 / 444
页数:5
相关论文
共 29 条
[1]   Use of recombinase gene fusions to identify Vibrio cholerae genes induced during infection [J].
Camilli, A ;
Mekalanos, JJ .
MOLECULAR MICROBIOLOGY, 1995, 18 (04) :671-683
[2]   USE OF GENETIC-RECOMBINATION AS A REPORTER OF GENE-EXPRESSION [J].
CAMILLI, A ;
BEATTIE, DT ;
MEKALANOS, JJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2634-2638
[3]   Differential patterns of acquired virulence genes distinguish Salmonella strains [J].
Conner, CP ;
Heithoff, DM ;
Julio, SM ;
Sinsheimer, RL ;
Mahan, MJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (08) :4641-4645
[4]  
Dawkins R., 1992, EXTENDED PHENOTYPE
[5]  
DYKHUIZEN DE, 1995, SYMP SOC GEN MICROBI, V52, P161
[6]   Identification of in vivo induced genes in Actinobacillus pleuropneumoniae [J].
Fuller, TE ;
Shea, RJ ;
Thacker, BJ ;
Mulks, MH .
MICROBIAL PATHOGENESIS, 1999, 27 (05) :311-327
[7]   The use of listeriolysin to identify in vivo induced genes in the Gram-positive intracellular pathogen Listeria monocytogenes [J].
Gahan, CGM ;
Hill, C .
MOLECULAR MICROBIOLOGY, 2000, 36 (02) :498-507
[8]  
GAL M, 1999, THESIS U OXFORD OXFO
[9]   IVIAT: a novel method to identify microbial genes expressed specifically during human infections [J].
Handfield, M ;
Brady, LJ ;
Progulske-Fox, A ;
Hillman, JD .
TRENDS IN MICROBIOLOGY, 2000, 8 (07) :336-339
[10]   Dissecting the biology of a pathogen during infection [J].
Heithoff, DM ;
Conner, CP ;
Mahan, MJ .
TRENDS IN MICROBIOLOGY, 1997, 5 (12) :509-513