Methylation as a crucial step in plant microRNA biogenesis

被引：812

作者：

Yu, B ^{[1
]}

Yang, ZY ^{[1
]}

Li, JJ ^{[1
]}

Minakhina, S ^{[1
]}

Yang, MC ^{[1
]}

Padgett, RW ^{[1
]}

Steward, R ^{[1
]}

Chen, XM ^{[1
]}

机构：

[1] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA

来源：

SCIENCE | 2005年 / 307卷 / 5711期

关键词：

D O I：

10.1126/science.1107130

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Methylation on the base or the ribose is prevalent in eukaryotic ribosomal RNAs (rRNAs) and is thought to be crucial for ribosome biogenesis and function. Artificially introduced 2'-O-methyl groups in small interfering RNAs (siRNAs) can stabilize siRNAs in serum without affecting their activities in RNA interference in mammalian cells. Here, we show that plant microRNAs (miRNAs) have a naturally occurring methyl group on the ribose of the last nucleotide. Whereas methylation of rRNAs depends on guide RNAs, the methyltransferase protein HEN1 is sufficient to methylate miRNA/miRNA* duplexes. Our studies uncover a new and crucial step in plant miRNA biogenesis and have profound implications in the function of miRNAs.

引用

页码：932 / 935

页数：4

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