Detection of infectious enteroviruses by an integrated cell culture PCR procedure

被引：93

作者：

Reynolds, KA

Gerba, CP

Pepper, IL

机构：

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 04期

关键词：

D O I：

10.1128/AEM.62.4.1424-1427.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rapid detection of infectious enteroviruses in environmental samples was made possible by utilizing an integrated cell culture-reverse transcriptase PCR approach. By this method, the presence of infectious enterovirus was confirmed within 24 h, compared,vith greater than or equal to 3 days by cell culture alone. The combined methodology eliminated typical problems normally associated with direct reverse transcriptase PCR by increasing the equivalent volume of environmental sample examined and reducing the effects of inhibitory compounds.

引用

收藏

页码：1424 / 1427

页数：4

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