Kinetics of growth of Aspergillus niger during submerged, agar surface and solid state fermentations

被引:42
作者
Favela-Torres, E [1 ]
Cordova-Lopez, J [1 ]
Garcia-Rivero, M [1 ]
Gutierrez-Rojas, M [1 ]
机构
[1] Univ Autonoma Metropolitana Iztapalapa, Dept Biotecnol, Mexico City 09340, DF, Mexico
关键词
Aspergillus niger; submerged culture; agar surface culture; solid state fermentation; kinetics;
D O I
10.1016/S0032-9592(97)00032-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effect of initial glucose concentration (30-450 g/litre) on the growth of Aspergillus niger 10 in submerged (SMF), agar surface (ASF) and solid state (SSF) fermentations was examined. Biomass production and specific growth rate were considerably less sensitive to changes in initial glucose concentration in SSF compared to either ASF or SMF. In all cases, maximum specific growth rates (0.323, 0.247 and 0.134 1/h for SSF, ASF and SMF, respectively) were obtained with the lowest initial glucose concentration employed (50 g/litre for SSF and 30 g/litre for both ASF and SMF). The rates of glucose uptake in SSF were considerably less affected by initial glucose concentrations when compared with ASF and SMF. Complete consumption of glucose in SSF, ASF and SMF was observed when glucose was applied at initial concentrations of 200, 150 and 100 g/litre, respectively. These results indicate that SSF is more suitable than either ASF or SMF for the growth of filamentous fungi, especially under conditions where catabolite repression applies (e.g. due to high glucose concentrations). (C) 1998 Elsevier Science Ltd.
引用
收藏
页码:103 / 107
页数:5
相关论文
共 19 条
[1]  
AURIA R, 1995, PROCESS BIOCHEM, V30, P751, DOI 10.1016/0032-9592(95)00004-F
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]  
Clutterbuck AJ, 1974, HDB GENETICS
[4]   Biomass estimation of Aspergillus niger growing on real and model supports in solid state fermentation [J].
CordovaLopez, J ;
GutierrezRojas, M ;
Huerta, S ;
SaucedoCastaneda, G ;
FavelaTorres, E .
BIOTECHNOLOGY TECHNIQUES, 1996, 10 (01) :1-6
[5]  
DESGRANGES C, 1991, APPL MICROBIOL BIOT, V35, P200
[6]   CITRIC-ACID AND POLYOLS PRODUCTION BY ASPERGILLUS-NIGER AT HIGH GLUCOSE-CONCENTRATION IN SOLID-STATE FERMENTATION ON INERT SUPPORT [J].
GUTIERREZROJAS, M ;
CORDOVA, J ;
AURIA, R ;
REVAH, S ;
FAVELATORRES, E .
BIOTECHNOLOGY LETTERS, 1995, 17 (02) :219-224
[7]   SOLID-STATE FERMENTATIONS [J].
HESSELTINE, CW .
BIOTECHNOLOGY AND BIOENGINEERING, 1972, 14 (04) :517-+
[8]   SCALE-UP STRATEGIES FOR SOLID-STATE FERMENTATION SYSTEMS [J].
LONSANE, BK ;
SAUCEDOCASTANEDA, G ;
RAIMBAULT, M ;
ROUSSOS, S ;
VINIEGRAGONZALEZ, G ;
GHILDYAL, NP ;
RAMAKRISHNA, M ;
KRISHNAIAH, MM .
PROCESS BIOCHEMISTRY, 1992, 27 (05) :259-273
[9]   PRODUCTION OF NEUTRAL PROTEASE BY MEMBRANE-SURFACE LIQUID CULTURE OF ASPERGILLUS-ORYZAE IAM2704 [J].
OGAWA, A ;
YASUHARA, A ;
TANAKA, T ;
SAKIYAMA, T ;
NAKANISHI, K .
JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 80 (01) :35-40
[10]  
Okasaki N., 1980, J FERMENT TECHNOL, V58, P471