Adaptive aberration correction in a two-photon microscope

被引：135

作者：

Neil, MAA

Juskaitis, R

Booth, MJ

Wilson, T

Tanaka, T

Kawata, S

机构：

[1] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England

[2] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan

来源：

JOURNAL OF MICROSCOPY | 2000年 / 200卷 / 02期

关键词：

adaptive aberration correction; confocal microscopy; two-photon microscopy;

D O I：

10.1046/j.1365-2818.2000.00770.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We demonstrate aberration correction in two-photon microscopy. Specimen-induced aberrations were measured with a modal wavefront sensor, implemented using a ferroelectric liquid crystal spatial light modulator (FLCSLM). Wavefront correction was performed using the same FLCSLM. Axial scanned (x-z) images of fluorescently labelled polystyrene beads using an oil immersion lens show restored sectioning ability at a depth of 28 mum in an aqueous specimen.

引用

页码：105 / 108

页数：4

共 19 条

[1] Smart microscope: an adaptive optics learning system for aberration correction in multiphoton confocal microscopy [J].