A closed conformation for the Pol λ catalytic cycle

被引：126

作者：

Garcia-Diaz, M

Bebenek, K

Krahn, JM

Kunkel, TA

Pedersen, LC

机构：

[1] NIEHS, Struct Biol Lab, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA

[2] NIEHS, Genet Mol Lab, NIH, Dept Hlth & Human Serv, Res Triangle Pk, NC 27709 USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2005年 / 12卷 / 01期

关键词：

D O I：

10.1038/nsmb876

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pollambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pollambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pollambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.

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页码：97 / 98

页数：2

相关论文

共 13 条

[1]

Bebenek K, 2004, ADV PROTEIN CHEM, V69, P137

[2] Structural and functional insights provided by crystal structures of DNA polymerases and their substrate complexes [J].

Brautigam, CA ;

Steitz, TA .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (01) :54-63

[3] STEREOCHEMISTRY OF INTERNUCLEOTIDE BOND FORMATION BY POLYNUCLEOTIDE PHOSPHORYLASE FROM MICROCOCCUS-LUTEUS [J].

BURGERS, PMJ ;

ECKSTEIN, F .

BIOCHEMISTRY, 1979, 18 (03) :450-454

[4] The mechanism of action of T7 DNA polymerase [J].

Doublié, S ;

Ellenberger, T .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 1998, 8 (06) :704-712

[5] A structural solution for the DNA polymerase λ-dependent repair of DNA gaps with minimal homology [J].

Garcia-Diaz, M ;

Bebenek, K ;

Krahn, JM ;

Blanco, L ;

Kunkel, TA ;

Pedersen, LC .

MOLECULAR CELL, 2004, 13 (04) :561-572

[6] Identification of an intrinsic 5′-deoxyribose-5-phosphate lyase activity in human DNA polymerase λ -: A possible role in base excision repair [J].

García-Díaz, M ;

Bebenek, K ;

Kunkel, TA ;

Blanco, L .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (37) :34659-34663

[7] DNA polymerase lambda (Pol λ), a novel eukaryotic DNA polymerase with a potential role in meiosis [J].

García-Díaz, M ;

Domínguez, O ;

López-Fernández, LA ;

de Lera, LT ;

Saníger, ML ;

Ruiz, JF ;

Párraga, M ;

García-Ortiz, MJ ;

Kirchhoff, T ;

del Mazo, J ;

Bernad, A ;

Blanco, L .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 301 (04) :851-867

[8] Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations [J].

Johnson, SJ ;

Taylor, JS ;

Beese, LS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3895-3900

[9] DNA replication fidelity [J].

Kunkel, TA ;

Bebenek, R .

ANNUAL REVIEW OF BIOCHEMISTRY, 2000, 69 :497-529

[10] Implication of DNA polymerase λ in alignment-based gap filling for nonhomologous DNA end joining in human nuclear extracts [J].

Lee, JW ;

Blanco, L ;

Zhou, T ;

Garcia-Diaz, M ;

Bebenek, K ;

Kunkel, TA ;

Wang, ZG ;

Povirk, LF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (01) :805-811