Interferon-beta and interferon-gamma synergistically inhibit the replication of severe acute respiratory syndrome-associated coronavirus (SARS-CoV)

被引：143

作者：

Sainz, B

Mossel, EC

Peters, CJ

Garry, RF

机构：

[1] Univ Texas, Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 USA

[2] Tulane Univ, Hlth Sci Ctr, Dept Microbiol & Immunol, Program Mol Pathogenesis & Immun, New Orleans, LA 70112 USA

[3] Univ Texas, Med Branch, Dept Pathol, Galveston, TX 77555 USA

来源：

VIROLOGY | 2004年 / 329卷 / 01期

关键词：

coronavirus; interferon-gamma; interferon-beta;

D O I：

10.1016/j.virol.2004.08.011

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent studies have shown that interferon-gamma (IFN-gamma) synergizes with IFN-alpha/beta to inhibit the replication of both RNA and DNA viruses. We investigated the effects of IFNs on the replication of two strains of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). While treatment of Vero E6 cells with 100 U/ml of either IFN-beta or IFN-gamma marginally reduced viral replication, treatment with both IFN-beta and IFN-gamma inhibited SARS-CoV plaque formation by 30-fold and replication by 3000-fold at 24 h and by > 1 X 10(5)-fold at 48 and 72 h post-infection. These studies suggest that combination IFN treatment warrants further investigation as a treatment for SARS. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：11 / 17

页数：7

共 20 条

[1] Inhibition of different lassa virus strains by alpha and gamma interferons and comparison with a less pathogenic arenavirus [J].

Asper, M ;

Sternsdorf, T ;

Hass, M ;

Drosten, C ;

Rhode, A ;

Schmitz, H ;

Günther, S .

JOURNAL OF VIROLOGY, 2004, 78 (06) :3162-3169

[2]

BERENBAUM MC, 1989, PHARMACOL REV, V41, P93

[3] Treatment of SARS with human interferons [J].

Cinatl, J ;

Morgenstern, B ;

Bauer, G ;

Chandra, P ;

Rabenau, H ;

Doerr, HW .

LANCET, 2003, 362 (9380) :293-294

[4] Identification of genes differentially regulated by interferon α, β, or γ using oligonucleotide arrays [J].

Der, SD ;

Zhou, AM ;

Williams, BRG ;

Silverman, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (26) :15623-15628

[5] Identification of a novel coronavirus in patients with severe acute respiratory syndrome [J].

Drosten, C ;

Günther, S ;

Preiser, W ;

van der Werf, S ;

Brodt, HR ;

Becker, S ;

Rabenau, H ;

Panning, M ;

Kolesnikova, L ;

Fouchier, RAM ;

Berger, A ;

Burguière, AM ;

Cinatl, J ;

Eickmann, M ;

Escriou, N ;

Grywna, K ;

Kramme, S ;

Manuguerra, JC ;

Müller, S ;

Rickerts, V ;

Stürmer, M ;

Vieth, S ;

Klenk, HD ;

Osterhaus, ADME ;

Schmitz, H ;

Doerr, HW .

NEW ENGLAND JOURNAL OF MEDICINE, 2003, 348 (20) :1967-1976

[6]

Fujii Takeshi, 2004, Journal of Infection and Chemotherapy, V10, P1, DOI 10.1007/s10156-003-0296-9

[7] Interferons: cell signalling, immune modulation, antiviral responses and virus countermeasures [J].

Goodbourn, S ;

Didcock, L ;

Randall, RE .

JOURNAL OF GENERAL VIROLOGY, 2000, 81 :2341-2364

[8] Isolation and characterization of viruses related to the SARS coronavirus from animals in Southern China [J].

Guan, Y ;

Zheng, BJ ;

He, YQ ;

Liu, XL ;

Zhuang, ZX ;

Cheung, CL ;

Luo, SW ;

Li, PH ;

Zhang, LJ ;

Guan, YJ ;

Butt, KM ;

Wong, KL ;

Chan, KW ;

Lim, W ;

Shortridge, KF ;

Yuen, KY ;

Peiris, JSM ;

Poon, LLM .

SCIENCE, 2003, 302 (5643) :276-278

[9] Pegylated interferon-α protects type 1 pneumocytes against SARS coronavirus infection in macaques [J].

Haagmans, BL ;

Kuiken, T ;

Martina, BE ;

Fouchier, RAM ;

Rimmelzwaan, GF ;

van Amerongen, G ;

van Riel, D ;

de Jong, T ;

Itamura, S ;

Chan, KH ;

Tashiro, M ;

Osterhaus, ADME .

NATURE MEDICINE, 2004, 10 (03) :290-293

[10] Interferon-β 1a and SARS coronavirus replication [J].

Hensley, LE ;

Fritz, EA ;

Jahrling, PB ;

Karp, CL ;

Huggins, JW ;

Geisbert, TW .

EMERGING INFECTIOUS DISEASES, 2004, 10 (02) :317-319

← 1 2 →