Simultaneous determination of theophylline and its metabolites by HPLC

被引：6

作者：

Kanazawa, H ^{[1
]}

Kizu, J ^{[1
]}

Matsushima, Y ^{[1
]}

机构：

[1] Kyoritsu Coll Pharm, Minato Ku, Tokyo 1058512, Japan

来源：

YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN | 2000年 / 120卷 / 10期

关键词：

HPLC; LC/MS; theophylline; caffeine; metabolite; column-switching system;

D O I：

10.1248/yakushi1947.120.10_1051

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A high performance liquid chromatography (HPLC) method has been developed for the simultaneous determination of theophylline and its metabolites, with caffeine and its metabolites. The method is simple and applicable to planning the administration of theophylline. We used a column-switching system in combination with atmospheric pressure chemical ionization liquid chromatography-mass spectrometry (LC-APCI-MS) for the determination of theophylline and its metabolites in biological samples. In the mass spectrum, the molecular ions of these drugs and metabolites were clearly observed as base peaks. The method is sufficiently sensitive and accurate for the pharmacokinetic studies of these drugs.

引用

页码：1051 / 1060

页数：10

共 27 条

[1] ENOXACIN - A POTENT INHIBITOR OF THEOPHYLLINE METABOLISM [J].