Structural and functional features of a specific nucleosome containing a recognition element for the thyroid hormone receptor

被引:70
作者
Wong, JM [1 ]
Li, Q [1 ]
Levi, BZ [1 ]
Shi, YB [1 ]
Wolffe, AP [1 ]
机构
[1] NICHHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA
关键词
chromatin structure; linker histones; nuclear receptors; nucleosome positioning; transcriptional regulation;
D O I
10.1093/emboj/16.23.7130
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Xenopus thyroid hormone receptor beta A (TR beta A) gene contains an important thyroid hormone response element (TRE) that is assembled into a positioned nucleosome. We determine the translational position of the nucleosome containing the TRE and the rotational positioning of the double helix with respect to the histone surface. Histone H1 is incorporated into the nucleosome leading to an asymmetric protection to micrococcal nuclease cleavage of linker DNA relative to the nucleosome core. Histone H1 association is without significant consequence for the binding of the heterodimer of thyroid hormone receptor and 9-cis retinoic acid receptor (TR/RXR) to nucleosomal DNA in vitro, or for the regulation of TR beta A gene transcription following microinjection into the oocyte nucleus. Small alterations of 3 and 6 bp in the translational positioning of the TRE in chromatin are also without effect on the transcriptional activity of the TR beta A gene, whereas a small change in the rotational position of the TRE (3 bp) relative to the histone surface significantly reduces the binding of TR/RXR to the nucleosome and decreases transcriptional activation directed by TR/RXR. Our results indicate that the specific architecture of the nucleosome containing the TRE may have regulatory significance for expression of the TR beta A gene.
引用
收藏
页码:7130 / 7145
页数:16
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共 80 条
  • [41] Evidence for a shared structural role for HMG1 and linker histones B4 and H1 in organizing chromatin
    Nightingale, K
    Dimitrov, S
    Reeves, R
    Wolffe, AP
    [J]. EMBO JOURNAL, 1996, 15 (03) : 548 - 561
  • [42] A single high affinity binding site for histone H1 in a nucleosome containing the Xenopus borealis 5 S ribosomal RNA gene
    Nightingale, KP
    Pruss, D
    Wolffe, AP
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (12) : 7090 - 7094
  • [43] The transcriptional coactivators p300 and CBP are histone acetyltransferases
    Ogryzko, VV
    Schiltz, RL
    Russanova, V
    Howard, BH
    Nakatani, Y
    [J]. CELL, 1996, 87 (05) : 953 - 959
  • [44] NUCLEOSOME ARRAYS INHIBIT BOTH INITIATION AND ELONGATION OF TRANSCRIPTS BY BACTERIOPHAGE-T7 RNA-POLYMERASE
    ONEILL, TE
    ROBERGE, M
    BRADBURY, EM
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1992, 223 (01) : 67 - 78
  • [45] SPECIFIC GLUCOCORTICOID RECEPTOR-BINDING TO DNA RECONSTITUTED IN A NUCLEOSOME
    PERLMANN, T
    WRANGE, O
    [J]. EMBO JOURNAL, 1988, 7 (10) : 3073 - 3079
  • [46] STRUCTURAL FEATURES OF A REGULATORY NUCLEOSOME
    PINA, B
    BARETTINO, D
    TRUSS, M
    BEATO, M
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 216 (04) : 975 - 990
  • [47] NUCLEOSOME POSITIONING MODULATES ACCESSIBILITY OF REGULATORY PROTEINS TO THE MOUSE MAMMARY-TUMOR VIRUS PROMOTER
    PINA, B
    BRUGGEMEIER, U
    BEATO, M
    [J]. CELL, 1990, 60 (05) : 719 - 731
  • [48] NUCLEOSOME RECONSTITUTION ON PLASMID-INSERTED POLY(DA).POLY(DT)
    PRUNELL, A
    [J]. EMBO JOURNAL, 1982, 1 (02) : 173 - 179
  • [49] HISTONE DNA CONTACTS IN A NUCLEOSOME CORE CONTAINING A XENOPUS 5S RIBOSOMAL-RNA GENE
    PRUSS, D
    WOLFFE, AP
    [J]. BIOCHEMISTRY, 1993, 32 (27) : 6810 - 6814
  • [50] An asymmetric model for the nucleosome: A binding site for linker histones inside the DNA gyres
    Pruss, D
    Bartholomew, B
    Persinger, J
    Hayes, J
    Arents, G
    Moudrianakis, EN
    Wolffe, AP
    [J]. SCIENCE, 1996, 274 (5287) : 614 - 617