Parallel nanoliter microfluidic analysis system

被引：87

作者：

Andersson, Per ^{[1
]}

Jesson, Gerald ^{[1
]}

Kylberg, Gunnar ^{[1
]}

Ekstrand, Gunnar ^{[1
]}

Thorsen, Gunnar ^{[1
]}

机构：

[1] Gyros AB, SE-75183 Uppsala, Sweden

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 11期

关键词：

D O I：

10.1021/ac061692y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A parallel nanoliter microfluidic analysis system based on capillary action, centrifugal force, and hydrophobic barriers is described. The precision of 112 parallel volume definition operations is determined to 0.75% CV at 200 nL using the individual sample introduction structure. For 20 nL, the actual measurement error is the dominating factor, with a combined error of 1.9% CV. Individual dispensing as well as dispensing through a common distribution channel is described. The volume definition precision for the common distribution channel is 1.6% CV for 200 nL. Unlike the dominating forces in microliter-sized channel systems, we describe hysteresis effects as exerting a major influence, which needs to be considered in order to control the operation and design of discrete nanoliter fluidics. Hydrophobic patches at the corners of the rectangular channel control corner-enhanced wicking. Excellent flow control of 1 and 2 nL/s is achieved using a predefined spin program.

引用

页码：4022 / 4030

页数：9

共 32 条

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