Occurrence of heparin in the invertebrate Styela plicata (Tunicata) is restricted to cell layers facing the outside environment -: An ancient role in defense?

被引:52
作者
Cavalcante, MCM
Allodi, S
Valente, AP
Straus, AH
Takahashi, HK
Mourao, PAS
Pavao, MSG
机构
[1] Univ Fed Rio de Janeiro, Ctr Ciencias Saude, Dept Bioquim Med, BR-21941590 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, HOsp Clementino Fraga Filho, Lab Tecido Conjuntivo, BR-21941590 Rio De Janeiro, Brazil
[3] Univ Fed Rio de Janeiro, Dept Histol & Embriol, Inst Ciencias Biomed, BR-21941590 Rio De Janeiro, Brazil
[4] Univ Fed Rio de Janeiro, Ctr Nacl Ressonancia Magnet Nucl Macromol, BR-21941590 Rio De Janeiro, Brazil
[5] Univ Fed Rio de Janeiro, Dept Bioquim Med, BR-21941590 Rio De Janeiro, Brazil
[6] Univ Fed Sao Paulo, Escola Paulista Med, Dept Bioquim, BR-04023900 Sao Paulo, Brazil
关键词
D O I
10.1074/jbc.M005830200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Heparin is an intracellular product of vertebrate mast cell currently used as exogenous anticoagulant. Despite of the potent biological activities of exogenous heparin, its physiological function has not been clearly established yet. Here, a heparin with similar structure and anticoagulant properties to the mammalian counterpart was shown to occur as the intracellular product of test cells, a cell monolayer that surrounds egg of the invertebrate Styela plicata (Chordata-Tunicata). As in the case of mammalian mast cells, heparin from the ascidian test cells is removed from the intracellular granules after incubation with compound 48/80, Following fertilization, the test cells surrounding the developing larva still retain heparin as metachromatic granulation. In the adult invertebrate, heparin occurs as intracellular granules at the apical tip of epithelial cells surrounding the lumen of both intestine and pharynx, in close contact with the external environment. This is the first description of the presence of heparin in cytoplasmic granules of epithelial-like cells around the lumen of sites exposed to external agents. This arrangement may reflect the participation of heparin in defense mechanisms in this invertebrate.
引用
收藏
页码:36189 / 36196
页数:8
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