Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior

被引：31

作者：

Bracey, E ^{[1
]}

Stenning, RA ^{[1
]}

Brooker, BE ^{[1
]}

机构：

[1] Food Res Inst, Reading Lab, Reading RG6 6BZ, Berks, England

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1998年 / 22卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

subtilisin; ultrasound irradiation; organic solvent; electron microscopy; particle size;

D O I：

10.1016/S0141-0229(97)00138-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems. Sonication was shown to be effective in reducing particle size, but the effect of any reduction in diffusional limitations on reaction rare was exceeded by an inhibitory effect of the treatment. This inhibition was found to be irreversible upon cessation of sonication and aqueous assay of activity, or recycling of the enzyme into a stirred reaction system. (C) 1998 Elsevier Science Inc.

引用

页码：147 / 151

页数：5

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