Characterization of grape procyanidins using high-performance liquid chromatography/mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：86

作者：

Yang, Y ^{[1
]}

Chien, MJ ^{[1
]}

机构：

[1] Givaudan Flavors Corp, Sci & Technol, Cincinnati, OH 45216 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 09期

关键词：

grape seed; catechin; procyanidins; HPLC/MS; MALDI-TOF MS;

D O I：

10.1021/jf000316q

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

High-performance liquid chromatography/mass spectrometry (HPLC/MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the procyanidin composition of the grape seed extract. The detection of the oligomers composed of (+)-catechin, (-)-epicatechin, and their galloylated derivatives in the grape seeds is demonstrated. With MALDI-TOF MS, oligomers up to nonamers were observed. The potential of the MALDI-TOF MS technique as a quantification tool is also discussed. The information presented in this study could lead to the determination of procyanidin content and their molecular weight distribution in grape seeds.

引用

页码：3990 / 3996

页数：7

共 48 条

[11]

3.0.CO

[12]

2-G

[13] Procyanidines from Vitis vinifera seeds protect rabbit heart from ischemia reperfusion injury: Antioxidant intervention and/or iron and copper sequestering ability [J].

Facino, RM ;

Carini, M ;

Aldini, G ;

Berti, F ;

Rossoni, G ;

Bombardelli, E ;

Morazzoni, P .

PLANTA MEDICA, 1996, 62 (06) :495-502

[14] Study of wine tannin oligomers by on-line liquid chromatography electrospray ionization mass spectrometry [J].

Fulcrand, H ;

Remy, S ;

Souquet, JM ;

Cheynier, V ;

Moutounet, M .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (03) :1023-1028

[15] Catechin and procyanidin composition of seeds from grape cultivars grown in Ontario [J].

Fuleki, T ;

Ricardo-da-Silva, JM .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (04) :1156-1160

[16] BEYOND ALCOHOL - BEVERAGE CONSUMPTION AND CARDIOVASCULAR MORTALITY [J].

GOLDBERG, DM ;

HAHN, SE ;

PARKES, JG .

CLINICA CHIMICA ACTA, 1995, 237 (1-2) :155-187

[17] Characterization of highly polymerized procyanidins in cider apple (Malus sylvestris var kermerrien) skin and pulp [J].

Guyot, S ;

Doco, T ;

Souquet, JM ;

Moutounet, M ;

Drilleau, JF .

PHYTOCHEMISTRY, 1997, 44 (02) :351-357

[18] Reversed-phase HPLC following thiolysis for quantitative estimation and characterization of the four main classes of phenolic compounds in different tissue zones of a French cider apple variety (Malus domestica var. Kermerrien) [J].

Guyot, S ;

Marnet, N ;

Laraba, D ;

Sanoner, P ;

Drilleau, JF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (05) :1698-1705

[19] High molecular weight plant polyphenolics (tannins) as biological antioxidants [J].

Hagerman, AE ;

Riedl, KM ;

Jones, GA ;

Sovik, KN ;

Ritchard, NT ;

Hartzfeld, PW ;

Riechel, TL .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1998, 46 (05) :1887-1892

[20] Identification of procyanidins in cocoa (Theobroma cacao) and chocolate using high-performance liquid chromatography mass spectrometry [J].

Hammerstone, JF ;

Lazarus, SA ;

Mitchell, AE ;

Rucker, R ;

Schmitz, HH .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (02) :490-496

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