Characterization of grape procyanidins using high-performance liquid chromatography/mass spectrometry and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：86

作者：

Yang, Y ^{[1
]}

Chien, MJ ^{[1
]}

机构：

[1] Givaudan Flavors Corp, Sci & Technol, Cincinnati, OH 45216 USA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 09期

关键词：

grape seed; catechin; procyanidins; HPLC/MS; MALDI-TOF MS;

D O I：

10.1021/jf000316q

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

High-performance liquid chromatography/mass spectrometry (HPLC/MS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) were used to characterize the procyanidin composition of the grape seed extract. The detection of the oligomers composed of (+)-catechin, (-)-epicatechin, and their galloylated derivatives in the grape seeds is demonstrated. With MALDI-TOF MS, oligomers up to nonamers were observed. The potential of the MALDI-TOF MS technique as a quantification tool is also discussed. The information presented in this study could lead to the determination of procyanidin content and their molecular weight distribution in grape seeds.

引用

页码：3990 / 3996

页数：7

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