Molecular genetic characterization and subcellular localization of a putative Theileria annulata membrane protein

被引:18
作者
Schneider, I [1 ]
Haller, D [1 ]
Seitzer, U [1 ]
Beyer, D [1 ]
Ahmed, JS [1 ]
机构
[1] Res Ctr Borstel, Div Vet Infectiol & Immunol, D-23845 Borstel, Germany
关键词
D O I
10.1007/s00436-004-1226-1
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A Theileria annulata protein (TaD) exhibiting an N-terminal signal sequence for endoplasmic reticulum membrane translocation and a conserved cysteine-rich region was isolated by screening the mRNA of a T. annulata-infected bovine lymphoblastoid cell line with degenerated primers directed against T. annulata-targeting sequences. The TaD-coding sequence was found to be most closely related to the genomic DNA sequence of T. parva ( TIGR database, 72%) and the amino acid sequence of Plasmodium falciparum (41%), P. yoelii yoelii (38%) and Cryptosporidium parvum (36%). The TaD mRNA is expressed within the sporozoite, schizont and merozoite stages of the parasite, implying that it is constitutively transcribed throughout the parasite's life cycle. Allelic variants were found between isolates originating from different geographical regions, however not affecting conserved cysteines. The open reading frame encoded a protein of 19.5 kDa and non-reducing SDS-PAGE analysis demonstrated a homodimeric protein. Using confocal microscopy, the protein was found to be both located in the parasite cytoplasm and to colocalize with a transmembrane protein of the schizonts within infected cells.
引用
收藏
页码:405 / 415
页数:11
相关论文
共 45 条
[12]   THEILERIA-PARVA INFECTION INDUCES AUTOCRINE GROWTH OF BOVINE LYMPHOCYTES [J].
DOBBELAERE, DAE ;
COQUERELLE, TM ;
RODITI, IJ ;
EICHHORN, M ;
WILLIAMS, RO .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (13) :4730-4734
[13]  
Fariselli P, 1999, PROTEINS, V36, P340
[14]  
Friedhoff K. T., 1997, Parassitologia (Rome), V39, P99
[15]   TRANSLOCATION OF PROTEINS ACROSS AND INTEGRATION OF MEMBRANE-PROTEINS INTO THE ROUGH ENDOPLASMIC-RETICULUM [J].
GILMORE, R ;
KELLARIS, KV .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1992, 674 :27-37
[16]   MIMICRY OF ELASTIN REPETITIVE MOTIFS BY THEILERIA-ANNULATA SPOROZOITE SURFACE-ANTIGEN [J].
HALL, R ;
HUNT, PD ;
CARRINGTON, M ;
SIMMONS, D ;
WILLIAMSON, S ;
MECHAM, RP ;
TAIT, A .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1992, 53 (1-2) :105-112
[17]   The PROSITE database, its status in 1999 [J].
Hofmann, K ;
Bucher, P ;
Falquet, L ;
Bairoch, A .
NUCLEIC ACIDS RESEARCH, 1999, 27 (01) :215-219
[18]  
Horton P, 1997, ISMB-97 - FIFTH INTERNATIONAL CONFERENCE ON INTELLIGENT SYSTEMS FOR MOLECULAR BIOLOGY, PROCEEDINGS, P147
[19]   FADING OF IMMUNOFLUORESCENCE DURING MICROSCOPY - A STUDY OF PHENOMENON AND ITS REMEDY [J].
JOHNSON, GD ;
DAVIDSON, RS ;
MCNAMEE, KC ;
RUSSELL, G ;
GOODWIN, D ;
HOLBOROW, EJ .
JOURNAL OF IMMUNOLOGICAL METHODS, 1982, 55 (02) :231-242
[20]   POLYMORPHISM OF SPAG-1, A CANDIDATE ANTIGEN FOR INCLUSION IN A SUBUNIT VACCINE AGAINST THEILERIA-ANNULATA [J].
KATZER, F ;
CARRINGTON, M ;
KNIGHT, P ;
WILLIAMSON, S ;
TAIT, A ;
MORRISON, IW ;
HALL, R .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 67 (01) :1-10